Project description:The sequence-specific transcription factors Ume6, Nrg1, Cin5, and Sok2 and the general transcription factor TFIIB mediate the genome-wide targeting of the ATP-dependent chromatin remodeling enzyme Isw2. At least two biological replicates comparing Isw2-ChIP (Isw2-K215R ChIP DNA competitively hybridized against WT Isw2 ChIP DNA) in WT and M-NM-^Tume6, M-NM-^Tnrg1, M-NM-^Tcin5, M-NM-^Tsok2, and sua7-1 strains or Ume6-ChIP (ChIP DNA competitively hybridized again Input DNA) in WT and sua7-1 strains
Project description:We report change in the chromatin contacts upon deletion of ATP-dependent chromatin remodellers (ISW1, ISW2 and CHD1) in Saccharomyces cerevisiae.
Project description:We report change in the nucleosome occupancy and accessibility upon deletion of ATP-dependent chromatin remodellers (ISW1, ISW2 & CHD1) in Saccharomyces cerevisiae.
Project description:This study addresses the effect of mutations related to sense and antisense transcription of Saccharomyces cerevisiae phosphate genes using strand-specific RNAseq. Replacement of the transcriptional terminator of PHO84 by that of CYC1 resulted, unexpectedly, in an increased antisense transcription and a strongly reduced sense transcription of PHO84. Also RNA levels of SPL2 were strongly reduced. Deletion of the PHO84 coding region did not markedly affect the expression of SPL2, suggesting that antisense transcription of PHO84 and not the Pho84 transporter affects the expression of SPL2. Deletion of the two putative binding sites for the transcriptional regulator Ume6 in the SPL2 promoter resulted in a slightly increased level of SPL2 RNA, whereas deletion of the UME6 gene resulted in a decreased level of SPL2 and PHO84 RNA. These results suggests that Ume6 is involved in the regulation of SPL2 by a mechanism different from a simple binding to the putative Ume6 binding sites.
Project description:We report change in the chromatin contacts at nucleosomal resolution upon deletion of ATP-dependent chromatin remodellers(Isw1,Isw2 and Chd1) in Saccharomyces cerevisiae.
Project description:The sequence-specific transcription factors Ume6, Nrg1, Cin5, and Sok2 and the general transcription factor TFIIB mediate the genome-wide targeting of the ATP-dependent chromatin remodeling enzyme Isw2.
Project description:Nucleosome positions were determined in wild type cells, cells lacking Isw2 or Ume6, and cells containing a hybrid Chd1-Ume6 chimeric remodeler
