Arabidopsis thaliana
Ontology highlight
ABSTRACT:
OTHER RELATED OMICS DATASETS IN: E-GEOD-40245
PROVIDER: PRJNA173332 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The goal of this experiment was to explore the molecular network of glucose-TOR signaling in Arabidopsis seedling autotrophic transition stage. We used the whole-genome microarrays to detail the global program of gene expression mediated by glucose and TOR.
2013-04-05 | GSE40245 | GEO
Project description:The goal of this experiment was to explore the molecular network of glucose-TOR signaling in Arabidopsis seedling autotrophic transition stage. We used the whole-genome microarrays to detail the global program of gene expression mediated by glucose and TOR. Arabidopsis WT and estradiol inducible RNAi-tor seedlings were germinated in low light condition for 3 days to deplete seed nutrient supply. Fifteen mM glucose was adding back to reactivate the quiescent seedlings and the samples were harvested for RNA isolation. Transcript profiles were analyzed and compared between with and without glucose treatment to identify early glucose-TOR target genes. Each experimental condition was repeated three times and total 12 samples were generated.
2013-04-05 | E-GEOD-40245 | biostudies-arrayexpress
Project description:There are two principal mechanisms underlie sugar perception and signal transduction in biological systems. Direct sensing and signaling is triggered by sugar direct binding sensors such as Hexokiase 1 (HXK1) with a broad range of affinity and specificity, and indirect sensing signaling via a variety of energy and metabolite sensor, such as Target of Rapamycin (TOR). To investigate the intertwined interaction between glucose and plant hormones ethylene, the transcriptome regulated by ethylene, glucose-TOR, and glucose-HXK1 were analyzed and compared. Our results unexpectedly revealed a deep antagonistic interaction between glucose-TOR-energy signaling and the plant stress hormone ethylene.
2020-09-08 | GSE104146 | GEO
Project description:The devastating blast fungus Magnaporthe oryzae elaborates invasive hyphae (IH) in living rice cells during early infection, separated from host cytoplasm by plant-derived interfacial membranes, but the metabolic strategies underpinning this fundamental intracellular biotrophic growth phase are poorly understood. Eukaryotic cell growth depends on activated target-of-rapamycin (TOR) kinase signaling, which inhibits autophagy. Here, using live-cell imaging coupled with multiomic approaches, we show how the M. oryzae serine/threonine protein kinase Rim15 coordinates cycles of autophagy and glutaminolysis in IH – the latter through phosphorylation of NAD-dependent glutamate dehydrogenase – to reactivate TOR and promote biotrophic growth. Deleting RIM15 attenuated IH growth and triggered plant immunity; these defects were fully remediated by exogenous α-ketoglutarate treatment, but glucose treatment only suppressed host defenses. Our results together suggest that Rim15-dependent cycles of autophagic flux liberate α-ketoglutarate – via glutaminolysis – to reactivate TOR signaling and fuel biotrophic growth while conserving glucose for antioxidation-mediated host innate immunity suppression.
2023-07-10 | PXD043076 | Pride
Project description:rs07-09_bou - catma1-bou - Autotrophic growth acquisition is abolished in the bou mutant in Arabidopsis thaliana. BOU encodes a putative mitochondrial acyl carnitine carrier. bou mutant is blocked at the cotyledon stage. Autotrophic growth of the bou mutant can be achieved with addition of sugar in the medium or in darkness. Moreover, BOU gene expression is activated by light and depends on plant developmental stage. We wish to determine what are the consequences of bou gene mutation at the transcriptome level. We wish to understand whether bou growth arrest is due to the modification of specific genes expression or to a general effect on metabolism at the transition from heterotrophic to autotrophic growth. - Seeds from a heterozygous plants were grown for either 5 or 8 days after germination on synthetic medium (MS/2) without sugar under continuous light. We harvested cotyledon-stage blocked plants (bou phenotype) from three independent Petri dishes and also green seedlings with true leaves and fully developed root (heterozygotes with a wild-type phenotype) . We also grew independently Col-O plants for 5 and 8 days to compare them with the bou mutants. Keywords: gene knock in (transgenic),normal vs disease comparison,time course
2009-02-05 | GSE10399 | GEO
Project description:In plants, sugars such as glucose act as signaling molecules that promote changes in gene expression programs impacting on growth and development. Recent evidences have revealed the potential participation of mRNA decay control in some aspects of glucose-mediated regulatory responses suggesting a role of microRNAs (miRNAs) in these responses. In order to get a better understanding of glucose-mediated development modulation involving miRNA-related regulatory pathways, early seedling development of mutants impaired in miRNA biogenesis (hyl1-2) and miRNA-activity (ago1-25) were evaluated. Both mutants exhibited a glucose hyposensitive phenotype from germination up to seedling establishment, indicating that miRNA regulatory pathways may be involved in the glucose-mediated delay of early seedling development. The expression profile of 200 primary miRNA transcripts (pri-miRs) were evaluated by large-scale quantitative real-time polymerase chain reaction (qRT-PCR) profiling revealing that 38 pri-miRs were regulated by glucose. For several of them, the corresponding mature miRNAs are known to participate directly or indirectly in plant development, and their accumulation was shown to be co-regulated with the pri-miRNA by glucose. These data indicate that deficiency in miRNA machinery leads to a deregulated expression of several miRNA target genes in response to glucose. Also, such deficiencies result in glucose-promoted misexpression of genes for the three Abscisic Acid signaling elements ABI3, ABI4 and ABI5. Thus, miRNA-regulatory pathways play a role in the adjustments of growth and development triggered by glucose signaling.
2013-09-06 | GSE43546 | GEO
Project description:In plants, sugars such as glucose act as signaling molecules that promote changes in gene expression programs impacting on growth and development. Recent evidences have revealed the potential participation of mRNA decay control in some aspects of glucose-mediated regulatory responses suggesting a role of microRNAs (miRNAs) in these responses. In order to get a better understanding of glucose-mediated development modulation involving miRNA-related regulatory pathways, early seedling development of mutants impaired in miRNA biogenesis (hyl1-2) and miRNA-activity (ago1-25) were evaluated. Both mutants exhibited a glucose hyposensitive phenotype from germination up to seedling establishment, indicating that miRNA regulatory pathways may be involved in the glucose-mediated delay of early seedling development. The expression profile of 200 primary miRNA transcripts (pri-miRs) were evaluated by large-scale quantitative real-time polymerase chain reaction (qRT-PCR) profiling revealing that 38 pri-miRs were regulated by glucose. For several of them, the corresponding mature miRNAs are known to participate directly or indirectly in plant development, and their accumulation was shown to be co-regulated with the pri-miRNA by glucose. These data indicate that deficiency in miRNA machinery leads to a deregulated expression of several miRNA target genes in response to glucose. Also, such deficiencies result in glucose-promoted misexpression of genes for the three Abscisic Acid signaling elements ABI3, ABI4 and ABI5. Thus, miRNA-regulatory pathways play a role in the adjustments of growth and development triggered by glucose signaling. Three samples, including untreated control, 2% glucose-treated and 2% mannitol-treated samples.
2013-09-06 | E-GEOD-43546 | biostudies-arrayexpress
Project description:We identified TOR target genes using three different TOR active site inhbitors AZD8055, TORIN1 and KU63749 in Arabidopsis thaliana shoots
2023-02-01 | GSE197099 | GEO
Project description:rs07-09_bou - catma1-bou - Autotrophic growth acquisition is abolished in the bou mutant in Arabidopsis thaliana. BOU encodes a putative mitochondrial acyl carnitine carrier. bou mutant is blocked at the cotyledon stage. Autotrophic growth of the bou mutant can be achieved with addition of sugar in the medium or in darkness. Moreover, BOU gene expression is activated by light and depends on plant developmental stage. We wish to determine what are the consequences of bou gene mutation at the transcriptome level. We wish to understand whether bou growth arrest is due to the modification of specific genes expression or to a general effect on metabolism at the transition from heterotrophic to autotrophic growth. - Seeds from a heterozygous plants were grown for either 5 or 8 days after germination on synthetic medium (MS/2) without sugar under continuous light. We harvested cotyledon-stage blocked plants (bou phenotype) from three independent Petri dishes and also green seedlings with true leaves and fully developed root (heterozygotes with a wild-type phenotype) . We also grew independently Col-O plants for 5 and 8 days to compare them with the bou mutants. Keywords: gene knock in (transgenic),normal vs disease comparison,time course 5 dye-swap - CATMA arrays
2009-02-05 | E-GEOD-10399 | biostudies-arrayexpress
Project description:This series contain all stages Arabidopsis plant development. Stages of development includes unfertilized ovule, 24-Hr post-fertilization seed, globular stage seed, cotyledon stage seed, mature green seed, post-mature green seed, post-germination seedling, rosette leaf, root, stem, and floral bud. Keywords = Arabidopsis Keywords = Seed development Keywords = seedling Keywords = germination Keywords = leaf Keywords = root Keywords = stem Keywords = floral bud Keywords: ordered
2005-01-01 | GSE680 | GEO