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ALKBH5 is a New Mammalian RNA Demethylase

ABSTRACT: ALKBH5 is a New Mammalian RNA Demethylase

PROVIDER: PRJNA173338 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR540252_1.fastq.gz	Fastqsanger.gz
	SRR540252_2.fastq.gz	Fastqsanger.gz
	SRR540253_1.fastq.gz	Fastqsanger.gz
	SRR540253_2.fastq.gz	Fastqsanger.gz
	SRR540254.fastq.gz	Fastqsanger.gz

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ALKBH5 is a New Mammalian RNA Demethylase

Project description:N6-methyladenosine (m6A) is the most prevalent internal modification of messenger RNA (mRNA) in higher eukaryotes. Here we report ALKBH5 as a new mammalian demethylase that oxidatively removes the m6A modification in mRNA in vitro and inside cells. This demethylation activity of ALKBH5 significantly affects mRNA export and RNA metabolism as well as the assembly of mRNA processing factors in nuclear speckles. Alkbh5-deficient male mice are characterized by impaired fertility resulting from apoptosis that affects meiotic metaphase-stage spermatocytes. In accordance with this defect, we have identified in mouse testes 1552 differentially expressed genes which cover broad functional categories and include spermatogenesis-related mRNAs involved in the p53 functional interaction network. We show that Alkbh5-deficiency impacts the expression levels of some of these mRNAs, supporting the observed phenotype. The discovery of this new RNA demethylase strongly suggests that the reversible m6A modification plays fundamental and broad functions in mammalian cells. RNA-seq in two cell types

2013-02-15 | E-GEOD-40132 | biostudies-arrayexpress

ALKBH5 is a New Mammalian RNA Demethylase

2013-02-15 | GSE40132 | GEO

ALKBH5 is a New Mammalian Nuclear RNA Demethylase that Affects RNA Metabolism

Project description:To identify ALKBH5-binding RNA, PAR-CLIP was performed to pull down ALKBH5-binding RNAs. After digestion with Rnase T1, small RNA-seq was performed. Through analysis of 50 million reads, we have found 933 ALKBH5-binding genes, among which mRNA accounts for 85%. further analysis revealed that ALKBH5 mainly binds to intron and exon region of mRNAs. among 933 genes,64 genes have been reported to be spermatogenesis related, and most of them are functionally related with p53 signaling. we have also found 9 consensus binding motifs based on the clusters significantly detected in RIP sample.

2015-06-01 | GSE38482 | GEO

RNA demethylase ALKBH5 promotes tumorigenesis in multiple myeloma [RNA-seq]

Project description:We here report ALKBH5, a m6A RNA demethylase, as a crucial oncogene in multiple myeloma (MM). Using various MM models, we demonstrated a critical requirement of ALKBH5 for MM cell proliferation in vitro and in vivo. RNA-seq was applied to compare the expression profile of MM cells with or without ALKBh5 knockdown.

2022-07-07 | GSE180214 | GEO

RNA demethylase ALKBH5 promotes tumorigenesis in multiple myeloma [m6A-seq]

Project description:We here report ALKBH5, a m6A RNA demethylase, as a crucial oncogene in multiple myeloma (MM). Using various MM models, we demonstrated a critical requirement of ALKBH5 for MM cell proliferation in vitro and in vivo. To identify the potential mRNA targets of ALKBH5, we conducted m6A-seq with mRNA samples enriched from MM cells with or without ALKBH5 knockdown.

2022-07-07 | GSE180207 | GEO

Chromatin state maintains human myeloid leukemia by regulating m6A demethylase ALKBH5 expression [RNA-Seq]

Project description:ALKBH5 is the RNA N(6)-methyladenosine (m6A) demethylase. To under sthand the function and mechnism of ALKBH5 in human acute myeloid leukemia, we compared the m6A profiling in wild-type, ALKBH5-knock-down, and ALKBH5 rescue THP1 cells.

2020-05-27 | GSE128574 | GEO

RNA demethylase ALKBH5 promotes tumorigenesis in multiple myeloma

Project description:RNA demethylase ALKBH5 promotes tumorigenesis in multiple myeloma

| PRJNA747029 | ENA

Chromatin state maintains human myeloid leukemia by regulating m6A demethylase ALKBH5 expression [m6A-seq]

2020-05-27 | GSE128520 | GEO

Chromatin state maintains human myeloid leukemia by regulating m6A demethylase ALKBH5 expression [Slam-seq]

Project description:ALKBH5 is the RNA N(6)-methyladenosine (m6A) demethylase. To understhand the function and mechnism of ALKBH5 in human acute myeloid leukemia, we compared the RNA decay rate in wild-type and ALKBH5-knock-down THP1 cells.

2020-05-27 | GSE146874 | GEO

Chromatin state maintains human myeloid leukemia by regulating m6A demethylase ALKBH5 expression [Ribo-seq]

Project description:ALKBH5 is the RNA N(6)-methyladenosine (m6A) demethylase. To understhand the function and mechnism of ALKBH5 in human acute myeloid leukemia, we compared the translational efficiency in wild-type and ALKBH5-knock-down THP1 cells.

2020-05-27 | GSE146849 | GEO

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