Project description:Interventions: experimental group:Orally take Combined Bifidobacterium, Lactobacillus, Enterococcus and Bacillus cereus Tablets;control group:Orally take placebo
Study Design: Parallel
Project description:The aim of the study was to carry out a CGH study utilizing a set of 39 diverse Bacillus isolates. Thirty four B. cereus and five B. anthracis strains and isolates were chosen so as to represent different lineages based on previous characterizations, including MLEE and MLST (Helgason, Okstad et al. 2000; Helgason, Tourasse et al. 2004). They represent the spectrum of B. cereus phenotypic diversity by including soil, dairy and periodontal isolates in addition to virulent B. anthracis strains.
Project description:Comparison of the Bacillus cereus with induced ComK1 (pATK31 in sample 1-3) or ComK2 (pATK32 in sample 4-6) proteins vs Bacillus cereus carrying empty plasmid (pLM5 in all samples)
Project description:Comparison of the Bacillus cereus with overexpressed Bacillus subtilis ComK (Bacillus cereus pNWcomKBsu) vs Bacillus cereus carrying empty plasmid (Bacillus cereus pNW33N) One condition design comparision of (IPTG induced overexpression construct vs IPTG induced empty plasmid) including a dye swap, 3 biological replicate
