Uncultured Vibrio sp.
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA175383 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Environmental isolates of Vibrio cholerae from California coastal water compared to reference strain N16961. A genotyping experiment design type classifies an individual or group of individuals on the basis of alleles, haplotypes, SNP's. Keywords: genotyping_design; array CGH
2007-07-17 | GSE8499 | GEO
Project description:Vibrio-specific 16S rRNA sequences from seawater, sediment, and oyster hatchery samples
| PRJNA402088 | ENA
Project description:Environmental isolates of Vibrio cholerae from California coastal water compared to reference strain N16961.
2007-05-23 | E-SMDB-4063 | biostudies-arrayexpress
Project description:Vibrio alginolyticus is a Gram-negative marine bacterium. A limited population of the organisms causes acute gastroenteritis in humans. In this study, Vibrio alginolyticus wild type strain EPGS is compared with the mutants of Ser-Thr kinase PpkA and phosphatase PppA, after cultured for 7h, in Luria-Bertani containing medium 3 % NaCl at 30 C. Our goal is to determine the ppkA and pppA regulon.
2011-12-31 | GSE24319 | GEO
Project description:The pathogenic marine bacterium Vibrio vulnificus is a causative agent of food-borne diseases such as life-threatening septicemia and gastroenteritis in individuals with underlying predisposed conditions.Because most infection cases of V. vulnificus are caused by ingestion of contaminated seafood, the pathogen has to encounter host cells in the gut at the early stage of infection and alters its gene expression patters to adapt the gut environment of host. To identify differentially expressed genes when V. vulnificus cells are exposed to host cells, a transcriptome analysis of V. vulnificus cells that were either exposed to HT29-methotrexate intestinal epithelial cells or grown in basal medium eagle (Sigma) (as a negative control) was performed using Illumina GAIIx system. 16S and 23S rRNA were depleted using MICROBExpress kit (Ambion) from the total RNAs of each sample. Strand-specific cDNA libraries of the samples were constructed using Truseq stranded mRNA prep. kit (Illumina). Single-end and 36 cycle sequencing were performed using Iluumina GAIIx sequencer by ChunLab (http://www.chunlab.com) and Raw sequence data were analyzed by CLC Genomics Workbench 5. 5. 1 (CLC Bio)
2017-08-29 | E-MTAB-5629 | biostudies-arrayexpress
Project description:Vibriosis caused by Vibrio vulnificus on eels represents an important threat for this specie under culture conditions. Development of new transcriptomic tools is essential to increase the knowledge of eel biology, that nowadays is scarcer. Therefore, using previous results obtained by 454 sequencing of the eel immune-enriched transcriptome, an eel-specific custom microarray have been designed. Gills transcriptomic pattern were analyzed as a principal portal of entry for pathogens in fish after 1h of bath infection with Vibrio vulnificus to describe gill immune response. Moreover, two different strains were used, vibro vulnificus wild type (R99) and rtx double mutant (CT285), to asses the virulence of these pathogen caused by MARTX.
2013-03-15 | GSE45163 | GEO
Project description:Vibrio alginolyticus is a Gram-negative marine bacterium. A limited population of the organisms causes acute gastroenteritis in humans. In this study, Vibrio alginolyticus wild type strain EPGS is compared with the mutants of Ser-Thr kinase PpkA and phosphatase PppA, after cultured for 7h, in Luria-Bertani containing medium 3 % NaCl at 30 C. Our goal is to determine the ppkA and pppA regulon. Three wild type and five mutant Vibrio alginolyticus samples were compared.
2011-12-31 | E-GEOD-24319 | biostudies-arrayexpress
Project description:Vibriosis caused by Vibrio vulnificus on eels represents an important threat for this specie under culture conditions. Development of new transcriptomic tools is essential to increase the knowledge of eel biology, that nowadays is scarcer. Therefore, using previous results obtained by 454 sequencing of the eel immune-enriched transcriptome, an eel-specific custom microarray have been designed. Gills transcriptomic pattern were analyzed as a principal portal of entry for pathogens in fish after 1h of bath infection with Vibrio vulnificus to describe gill immune response. Moreover, two different strains were used, vibro vulnificus wild type (R99) and rtx double mutant (CT285), to asses the virulence of these pathogen caused by MARTX. Adult european eels were bath infected with two Vibrio vulnificus strains, the wild type and double Rtx mutant (CT285). After 0, 3, 12h post-infection eel gills were sampled. Three individuals per experimental point were sampled, including a Control group and a Handling control group. Obtaining a total of 24 samples. The transcriptomic profile was described for each individual sample.
2013-03-15 | E-GEOD-45163 | biostudies-arrayexpress
Project description:To investigate the effect of conditions of culture on gene expression in Vibrio cholerae N16961 We then performed gene expression profiling analysis using data obtained from RNA-seq of 4 conditions of culture in biological duplicate
2023-02-20 | GSE214813 | GEO