Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA178531 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Dietary glucosylceramide (GC) improves skin barrier function. To elucidate the molecular mechanisms involved, we used a microarray system to analyze mRNA expression in SDS-treated dorsal skin of hairless mouse. Transepidermal water loss of mouse skin was increased by SDS treatment and the increase was significantly reduced by prior oral administration of glucosylceramides. Microarray-evaluated mRNA expression ratios showed statistically significant increase of expression of genes related to cornified envelope and tight junction formation versus all genes in glucosylceramide-fed/SDS-treated mouse skin. We then examined the contribution of glucosylceramide metabolites to tight junction formation of cultured keratinocytes. SDS treatment of cultured keratinocytes significantly decreased the transepidermal electrical resistance, and the decrease was significantly ameliorated in the presence of sphingosine or phytosphingosine, the major metabolites of glucosylceramide. These results suggest that oral administration of glucosylceramide improves skin barrier function by upregulating genes associated with both cornified envelope and tight junction formation. Two-condition experiment, effect of oral intake of GC on mice skin before SDS treatment (0d), and after SDS treatment(2d), Biological replicates: 3 replicates for 0d, 4 replicates for 2d.
2012-03-31 | E-GEOD-28086 | biostudies-arrayexpress
Project description:To identify Ras, MEK and ERK target genes which regulate tight junction formation in 16HBE bronchial epithelial cells
2018-12-01 | GSE63229 | GEO
Project description:Most tumors are epithelial-derived, and although disruption of polarity and aberrant cellular junction formation is a poor prognosticator in human cancer, the role of polarity determinants in oncogenesis is poorly understood. Using in vivo selection, we identified a mammalian orthologue of the Drosophila polarity regulator crumbs as a gene whose loss of expression promotes tumor progression. Immortal baby mouse kidney epithelial (iBMK) cells selected in vivo to acquire tumorigenicity displayed dramatic repression of crumbs3 (crb3) expression associated with disruption of tight junction formation, apicobasal polarity, and contact-inhibited growth. Restoration of crb3 expression restored junctions, polarity and contact inhibition, while suppressing migration and metastasis. These findings suggest a role for mammalian polarity determinants in suppressing tumorigenesis that may be analogous to the well-studied polarity tumor suppressor mechanisms in Drosophila. Keywords: Crumbs, crb3, tight junctions, polarity, metastasis, cancer
2008-03-25 | GSE10682 | GEO
Project description:Time-resolve proximity proteomics of tight junction. To understand how the tight junction belt is assembled and positioned, we combined APEX2 proximity proteomics of the main junctional scaffold protein ZO-1 with a calcium switch tissue formation assay.This combination allowed us to synchronize the initiation of junction assembly in the entire tissue by the addition of calcium to the culture medium and quantify the time evolution of the junctional proteome during the assembly process using proximity proteomics.
2024-05-22 | PXD052221 | Pride
Project description:Dietary glucosylceramide (GC) improves skin barrier function. To elucidate the molecular mechanisms involved, we used a microarray system to analyze mRNA expression in SDS-treated dorsal skin of hairless mouse. Transepidermal water loss of mouse skin was increased by SDS treatment and the increase was significantly reduced by prior oral administration of glucosylceramides. Microarray-evaluated mRNA expression ratios showed statistically significant increase of expression of genes related to cornified envelope and tight junction formation versus all genes in glucosylceramide-fed/SDS-treated mouse skin. We then examined the contribution of glucosylceramide metabolites to tight junction formation of cultured keratinocytes. SDS treatment of cultured keratinocytes significantly decreased the transepidermal electrical resistance, and the decrease was significantly ameliorated in the presence of sphingosine or phytosphingosine, the major metabolites of glucosylceramide. These results suggest that oral administration of glucosylceramide improves skin barrier function by upregulating genes associated with both cornified envelope and tight junction formation.
2012-04-01 | GSE28086 | GEO
Project description:Epithelium barrier integrity is assumed at least in part by the presence of junctions between cells. Tight-junction formation may be necessary for Sertoli cells to cease dividing and to support germ cell development. Microarray analysis of P20 Cldn11 +/- and Cldn11-/- testes confirmed the impact od Cldn11 deficiency on cell cytoskeleton and junction-related genes. Keywords: transcriptomic analysis
2010-04-11 | GSE15492 | GEO
Project description:Clostridioides difficile toxins deregulate tight junction proteins.
| PRJNA973611 | ENA
Project description:c-Jun is a gene expression regulator. By forming homo- or heterodimer, c-Jun binds to DNA and regulates gene transcription. c-Jun is deeply involved in embryonic development but its effect on nervous system development especially in higher mammal is unclear. In this study, we combined H1ESC derived neural progenitor cells (NPCs), cerebral organoids (COs),Thalamus Organoids(ThOs) and mouse model to study the role of c-Jun in early nervous system development. c-Jun KO promoted NPCs induction and differentiation while weakened NPCs’ adhesion ability. c-Jun KO COs got more robust neural ectoderm and expanded Pax6+/Nestin+ cortex-like layer while less tight junctional core. c-Jun KO mouse embryos on E14.5 showed malformation of thalamus in diencephalon with tight junction loose and cell lose. Taken together, the consistent trend in three models implied that c-Jun deletion promoted neural differentiation while weakened the tight junctions. The thalamus/diencephalon was vulnerable to dysplasia when lose c-Jun in early mouse embryonic development.
2024-12-20 | GSE260732 | GEO
Project description:c-Jun is a gene expression regulator. By forming homo- or heterodimer, c-Jun binds to DNA and regulates gene transcription. c-Jun is deeply involved in embryonic development but its effect on nervous system development especially in higher mammal is unclear. In this study, we combined H1ESC derived neural progenitor cells (NPCs), cerebral organoids (COs) and mouse model to study the role of c-Jun in early nervous system development. c-Jun KO promoted NPCs induction and differentiation while weakened NPCs’ adhesion ability. c-Jun KO COs got more robust neural ectoderm and expanded Pax6+/Nestin+ cortex-like layer while less tight junctional core. c-Jun KO mouse embryos on E14.5 showed malformation of thalamus in diencephalon with tight junction loose and cell lose. Taken together, the consistent trend in three models implied that c-Jun deletion promoted neural differentiation while weakened the tight junctions. The thalamus/diencephalon was vulnerable to dysplasia when lose c-Jun in early mouse embryonic development.
2024-12-20 | GSE243735 | GEO
Project description:Object. Detailed and exact mechanisms underlying brain arteriovenous malformations (bAVM) are still confusing in clinic. Understanding the quantitative changes of proteins and signaling pathways would provide useful information for clinicians to understand the formation and development of bAVM and therefore give proper individual treatment strategies. This study was performed to establish a huge human bAVM proteome database by tandem mass tag (TMT)-labeling technique and detect the alteration of proteins and pathways in the development of human bAVM. Methods. This study used quantitative proteomics to profile protein changes with the 6-plex TMT labeling in bAVM lesions. Integrated bioinformatics analysis were used to classify and identify the altered proteins and relating signaling pathways. Western blot analyses were used to identify the reliability of the proteomic data. Results. Our work established one of the largest human bAVM proteome databases to date. A total of 1264 proteins were identified, among which the expression of 316 proteins were changed with 249 proteins upregulated. Bioinformatics analysis revealed a close relevance between cell-cell interactions including focal adhesion, tight junction, gap junction and the development of bAVM. Conclusions. Cell-cell interactions, including focal adhesion, tight junction and gap junction played vital roles in the formation and development of bAVM. Understanding the molecular mechanism underlying bAVM is important for the development of future therapeutic approaches, thereafter making precise and individual treatment strategies in clinic.
2017-03-29 | MSV000080716 | MassIVE