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Membrane prewetting by condensates promotes tight junction belt formation

ABSTRACT: Time-resolve proximity proteomics of tight junction. To understand how the tight junction belt is assembled and positioned, we combined APEX2 proximity proteomics of the main junctional scaffold protein ZO-1 with a calcium switch tissue formation assay.This combination allowed us to synchronize the initiation of junction assembly in the entire tissue by the addition of calcium to the culture medium and quantify the time evolution of the junctional proteome during the assembly process using proximity proteomics.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Canis Familiaris (dog) (canis Lupus Familiaris)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Karina pombo garcia

LAB HEAD: karina pombo garcia

PROVIDER: PXD052221 | Pride | 2024-05-22

REPOSITORIES: Pride

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Membrane prewetting by condensates promotes tight-junction belt formation.

Pombo-García Karina K Adame-Arana Omar O Martin-Lemaitre Cecilie C Jülicher Frank F Honigmann Alf A

Nature 20240807 8025

Biomolecular condensates enable cell compartmentalization by acting as membraneless organelles<sup>1</sup>. How cells control the interactions of condensates with other cellular structures such as membranes to drive morphological transitions remains poorly understood. We discovered that formation of a tight-junction belt, which is essential for sealing epithelial tissues, is driven by a wetting phenomenon that promotes the growth of a condensed ZO-1 layer<sup>2</sup> around the apical membrane i ...[more]

PMID: 39112699

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