Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Metschnikowia pulcherrima 277

ABSTRACT: Metschnikowia pulcherrima 277 Genome sequencing and assembly

PROVIDER: PRJNA179032 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	ANFW02.dat.gz	Other
	ANFW02.fasta.gz	Fasta.gz
	ANFW02.master.dat	Other
	SRR2911418_1.fastq.gz	Fastqsanger.gz
	SRR2911418_2.fastq.gz	Fastqsanger.gz

Items per page:

1 - 5 of 6

Similar Datasets

Metschnikowia pulcherrima 277

Project description:Metschnikowia pulcherrima 277 Transcriptome or Gene expression

| PRJNA168317 | ENA

Metschnikowia pulcherrima

Project description:Metschnikowia pulcherrima overview

| PRJNA524221 | ENA

Metschnikowia pulcherrima strain:Bath1

Project description:Metschnikowia pulcherrima strain:Bath1 Genome sequencing and assembly

| PRJNA389552 | ENA

Metschnikowia pulcherrima strain:DBT012

Project description:Metschnikowia pulcherrima strain:DBT012 Genome sequencing and assembly

| PRJNA908754 | ENA

Metschnikowia pulcherrima strain:FL01

Project description:Metschnikowia pulcherrima strain:FL01 Genome sequencing and assembly

| PRJNA549352 | ENA

Metschnikowia aff. pulcherrima

Project description:Metschnikowia aff. pulcherrima overview

| PRJNA525933 | ENA

RNA-seq analysis of wt Metschnikowia pulcherrima cells and a spontaneous mutant (W8) in the snf2 gene that exhibited differential expression of the recently described pulcherrimin genes

Project description:We analyzed differential gene expression in wt and a snf2 mutant (W8) cells; the pucherimin biosynthesis genes were among the top diff. regulated genes (reduced expression in the mutant). ABSTRACT: Metschnikowia pulcherrima synthesizes the pigment pulcherrimin, from cyclodileucine (cyclo(Leu-Leu)) as a precursor, and exhibits strong antifungal activity against notorious plant pathogenic fungi. This yeast therefore has great potential for biocontrol applications against fungal diseases; particularly in the phyllosphere where this species is frequently found. To elucidate the molecular basis of the antifungal activity of M. pulcherrima, we compared a wildtype strain with a spontaneously occurring, pigmentless, weakly antagonistic mutant derivative. Whole genome sequencing of the wildtype and mutant strains identified a point mutation that creates a premature stop codon in the transcriptional regulator SNF2 in the mutant strain. Complementation of the mutant strain with the wildtype SNF2 gene restored pigmentation and recovered the strong antifungal activity. Mass spectrometry (UPLC HR HESI-MS) proved the presence of the pulcherrimin precursors cyclo(Leu-Leu) and pulcherriminic acid and identified new precursor and degradation products of pulcherriminic acid and/or pulcherrimin. All of these compounds were identified in the wildtype and complemented strain, but were undetectable in the pigmentless snf2 mutant strain. These results thus identify Snf2 as a regulator of antifungal activity and pulcherriminic acid biosynthesis in M. pulcherrima and provide a starting point for deciphering the molecular functions underlying the antagonistic activity of this yeast.

2019-09-01 | GSE128386 | GEO

Metschnikowia aff. pulcherrima strain:UCD127

Project description:Metschnikowia aff. pulcherrima strain:UCD127 Genome sequencing and assembly

| PRJNA449685 | ENA

Metschnikowia pulcherrima isolate:MS612

Project description:Metschnikowia pulcherrima MS612 Raw sequence reads

| PRJNA682923 | ENA

Metschnikowia aff. pulcherrima strain:APC 1.2

Project description:Metschnikowia aff. pulcherrima strain:APC 1.2 Genome sequencing

| PRJNA508581 | ENA