Project description:Investigation of gene expression level changes in Gordonia sp. KTR9 upon exposure to RDX and Nitrogen Limitation, compared to controls with no RDX. The Gordonia sp. KTR9 strain used in this study has been previously described by Thompson KT, Crocker FH, Fredrickson HL.2005. Mineralization of the cyclic nitramine explosive hexahydro-1,3,5-trinitro-1,3,5-triazine by Gordonia and Williamsia spp. Appl Environ Microbiol. 2005 Dec;71(12):8265-72.
Project description:Investigation of gene expression level changes in Gordonia sp. KTR9 upon exposure to RDX and Nitrogen Limitation, compared to controls with no RDX. The Gordonia sp. KTR9 strain used in this study has been previously described by Thompson KT, Crocker FH, Fredrickson HL.2005. Mineralization of the cyclic nitramine explosive hexahydro-1,3,5-trinitro-1,3,5-triazine by Gordonia and Williamsia spp. Appl Environ Microbiol. 2005 Dec;71(12):8265-72. A 12 x 135K array study using total RNA recovered from triplicate cultures of KTR9 exposed to RDX, triplicate cultures of KTR9 exposed to RDX and high nitrogen conditions, triplicate cultures of KTR9 exposed to low nitrogen, and triplicate cultures of controls exposed to high nitrogen.
Project description:Investigation of gene expression level changes in Gordonia sp. KTR9 and Gordonia sp. KTR9 mutant GlnR upon exposure to high and low nitrogen conditions The Gordonia sp. KTR9 strain used in this study has been previously described by Thompson KT, Crocker FH, Fredrickson HL.2005. Mineralization of the cyclic nitramine explosive hexahydro-1,3,5-trinitro-1,3,5-triazine by Gordonia and Williamsia spp. Appl Environ Microbiol. 2005 Dec;71(12):8265-72.
Project description:Investigation of gene expression level changes in Gordonia sp. KTR9 and Gordonia sp. KTR9 mutant GlnR upon exposure to high and low nitrogen conditions The Gordonia sp. KTR9 strain used in this study has been previously described by Thompson KT, Crocker FH, Fredrickson HL.2005. Mineralization of the cyclic nitramine explosive hexahydro-1,3,5-trinitro-1,3,5-triazine by Gordonia and Williamsia spp. Appl Environ Microbiol. 2005 Dec;71(12):8265-72. A 12 x 135K array study using total RNA recovered from triplicate cultures of KTR9 exposed to high nitrogen conditions, triplicate cultures of KTR9 exposed to low nitrogen conditions, triplicate cultures of KTR9 mutant GlnR exposed to high nitrogen conditions, triplicate cultures of KTR9 mutant GlnR exposed to low nitrogen conditions.
Project description:To further explore potential molecular mechanisms and pathways by which the presence or absence of the pGKT2 plasmid may be affecting the overall fitness cost in the native Gordonia sp KTR9 strain, transcriptome studies were performed. Transcriptome experiments comparing KTR9 wild-type and mutant strains grown in rich media confirmed the loss of the pGKT2 plasmid and also indicated the loss of the 90 kb pGKT1 plasmid.
Project description:Gene disruption of KTR9_0186 resulted in a 2-fold increase in TAG content in nitrogen starved cells. Lipase mutants subjected to carbon starvation, following nitrogen starvation, retained 75% more TAGs and retained pigmentation. Transcriptome expression data confirmed the deletion of KTR9_0186 and identified the up-regulation of key genes involved in fatty acid degradation, a likely compensatory mechanism for reduced TAG mobilization. The Gordonia sp. KTR9 strain used in this study has been previously described (PMID 16332812)
Project description:Gene disruption of KTR9_0186 resulted in a 2-fold increase in TAG content in nitrogen starved cells. Lipase mutants subjected to carbon starvation, following nitrogen starvation, retained 75% more TAGs and retained pigmentation. Transcriptome expression data confirmed the deletion of KTR9_0186 and identified the up-regulation of key genes involved in fatty acid degradation, a likely compensatory mechanism for reduced TAG mobilization. The Gordonia sp. KTR9 strain used in this study has been previously described (PMID 16332812) A 12 x 135K array study using total RNA recovered from triplicate cultures of KTR9 under carbon starvation and triplicate cultures of KTR9 0186 Mutant under carbon starvation.
Project description:The effects of putative lipase and wax ester synthase/acyl-CoA:diacylglycerol acyltransferase gene knockouts on triacylglycerol accumulation and transcriptome expression in Gordonia sp. KTR9.
