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Mus musculus

ABSTRACT: Genome-wide identification of Ikaros binding sites in primary pre-B cells

PROVIDER: PRJNA182016 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR619469.fastq.gz	Fastqsanger.gz
	SRR619470.fastq.gz	Fastqsanger.gz
	SRR619471.fastq.gz	Fastqsanger.gz
	SRR619472.fastq.gz	Fastqsanger.gz
	SRR619473.fastq.gz	Fastqsanger.gz

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Mus musculus

Project description:Genome-wide identification of Ikaros binding sites in pre-B cells

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Mus musculus

Project description:Genome-wide mapping of EBF1 binding sites in murine pre B-cells

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Genome-wide identification of Ikaros binding sites in primary pre-B cells

Project description:Ikaros DNA binding proteins are important regulators of haematopoiesis and genetic deletion of Ikaros results in severe developmental disturbances, including delayed thymocyte differentiation and an early and complete block in B cell development. Although Ikaros ChIP-seq data are available for mouse thymocytes and human haematopoietic progenitors, it has not been achieved in B cell progenitors. The goal of this study was to identify Ikaros binding sites in pre-B cells to define Ikaros target genes which could explain the essential role of Ikaros proteins in B cell differentiation. We carried out chromatin immunoprecipitation and high throughput sequencing (ChIP-seq) with antibodies to the C-terminus of endogenous Ikaros in primary pre-B cells isolated from mouse fetal livers.

2013-01-15 | E-GEOD-42462 | biostudies-arrayexpress

Mus musculus

Project description:Gene expression in mouse primary pre-B cells transduced with Ikaros

| PRJNA182250 | ENA

Gene expression in mouse primary pre-B cells transduced with Ikaros

Project description:Ikaros family DNA binding proteins are critical regulators of B cell development. To identify Ikaros-regulated genes in primary pre-B cells we performed gene expression microarrays. We used retroviral gene transfer to express Ikaros proteins. Total RNA from 3 biological replicates of primary pre-B cells transduced with wild type Ikaros (HA-Ikaros-IRES-GFP) and control vector (IRES-GFP) was isolated 48h after infection.

2013-01-15 | E-GEOD-42551 | biostudies-arrayexpress

Mus musculus

Project description:Identification of Lhx5 binding sites

| PRJNA261492 | ENA

Mus musculus

Project description:FOXL2 binding sites in primary cells from mouse ovarian follicles

| PRJNA259676 | ENA

Gene expression in mouse primary pre-B cells transduced with Ikaros

2013-01-15 | GSE42551 | GEO

Mus musculus

Project description:Identification and Characterization of Hoxa9 Binding Sites in Hematopoietic Cells

| PRJNA154277 | ENA

Genome wide mapping of IKAROS binding (ChIP-Seq) in human patient-derived BCR-ABL1+ (Ph+) pre-B ALL xenograft cells

Project description:IKAROS is an important tumor suppressor in human pre-B ALL and is mutated or deleted in a high percentage of human BCR-ABL1+ (Ph+) pre-B ALL. We here report the genome-wide binding of IKAROS in two independent patient-derived BCR-ABL1+ (Ph+) pre-B ALL xenograft cells that express wild type full-length IKAROS.

2017-02-06 | GSE58825 | GEO

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