Toxoplasma gondii
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ABSTRACT:
PROVIDER: PRJNA185412 | ENA |
REPOSITORIES: ENA
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Project description:Coordinated Progression Through Two Subtranscriptomes Underlies the Tachyzoite Cycle of Toxoplasma gondii
| PRJNA120513 | ENA
Project description:Toxoplasma proliferation is governed by its flexible but tightly regulated cell division cycle, that remarkably depends on appropriate and temporal transcriptional waves throughout the division cycle although the underlying regulation network is still unclear. Here we reported the transcriptional factor, AP2XI-3, as an essential cell cycle related gene regulator in G1 progression. After AP2XI-3 depletion, tachyzoites growth was obviously arrested in G1 phage and daughter budding progression was also destroyed. Intriguingly, the growth arrest of mutant parasites was also observed in vitro. Combining analysis of RNA sequencing and Cut & TAG revealed the key regulatory role of AP2XI-3 in G1 phage related biomass accumulation and cellular competent. AP2XI-3 controlled and targeted a set of RNA transcription, metabolism or protein biosynthesis related genes, whose dysregulation functionally disrupted cell homeostasis and parasite division. Altogether, our finding demonstrated the functional role of AP2XI-3 in transcriptional controlling of G1 progression and revealed its potential ability as a drug design target.
2026-01-01 | GSE285354 | GEO
Project description:Toxoplasma proliferation is governed by its flexible but tightly regulated cell division cycle, that remarkably depends on appropriate and temporal transcriptional waves throughout the division cycle although the underlying regulation network is still unclear. Here we reported the transcriptional factor, AP2XI-3, as an essential cell cycle related gene regulator in G1 progression. After AP2XI-3 depletion, tachyzoites growth was obviously arrested in G1 phage and daughter budding progression was also destroyed. Intriguingly, the growth arrest of mutant parasites was also observed in vitro. Combining analysis of RNA sequencing and Cut & TAG revealed the key regulatory role of AP2XI-3 in G1 phage related biomass accumulation and cellular competent. AP2XI-3 controlled and targeted a set of RNA transcription, metabolism or protein biosynthesis related genes, whose dysregulation functionally disrupted cell homeostasis and parasite division. Altogether, our finding demonstrated the functional role of AP2XI-3 in transcriptional controlling of G1 progression and revealed its potential ability as a drug design target.
2026-01-01 | GSE304633 | GEO
Project description:Toxoplasma proliferation is governed by its flexible but tightly regulated cell division cycle, that remarkably depends on appropriate and temporal transcriptional waves throughout the division cycle although the underlying regulation network is still unclear. Here we reported the transcriptional factor, AP2XI-3, as an essential cell cycle related gene regulator in G1 progression. After AP2XI-3 depletion, tachyzoites growth was obviously arrested in G1 phage and daughter budding progression was also destroyed. Intriguingly, the growth arrest of mutant parasites was also observed in vitro. Combining analysis of RNA sequencing and Cut & TAG revealed the key regulatory role of AP2XI-3 in G1 phage related biomass accumulation and cellular competent. AP2XI-3 controlled and targeted a set of RNA transcription, metabolism or protein biosynthesis related genes, whose dysregulation functionally disrupted cell homeostasis and parasite division. Altogether, our finding demonstrated the functional role of AP2XI-3 in transcriptional controlling of G1 progression and revealed its potential ability as a drug design target.
2026-01-01 | GSE285353 | GEO
Project description:Global transcriptome analysis indicates that depletion of rps13 and ribosomes leads to cell cycle arrest in G1
| PRJNA124799 | ENA
Project description:Cell cycle-dependent, intercellular transmission of Toxoplasma gondii is accompanied by marked changes in parasite gene expression.
| PRJNA125167 | ENA
Project description:A nucleolar AAA-NTPase is required for Toxoplasma gondii division
| PRJNA187427 | ENA
Project description:Cactin is essential for G1 progression in Toxoplasma gondii
| PRJNA153241 | ENA
Project description:Cyst formation is a key feature of the T. gondii life cycle but the genetic networks that drive this process are not yet fully characterized. To identify new components of this network, we compared T. gondii to its nearest extant relative Hammondia hammondi given the critical differences between these species in the timing and efficiency of cyst formation. Using transcriptional data from critical developmental and pH exposure time points from both species, we identified the gene TGVEG_311100, which we named Regulator of Cystogenesis 1 (ROCY1), as being both necessary and sufficient for cyst formation in T. gondii. Compared to WT parasites, TGVEG?ROCY1 parasites formed significantly fewer tissue cysts in response to alkaline pH stress in vitro and cysts were nearly undetectable in mouse brains for up to 9 weeks post-infection. Overexpression of tagged ROCY1 in WT parasites was sufficient to induce cyst formation in vitro in both WT and ROCY1-deficient parasites, demonstrating that ROCY1 is both necessary and sufficient for cyst formation. Moreover this induction of cyst formation required at least 1 of 3 predicted CCCH Zinc finger domains. Mice chronically infected with ?ROCY1 parasites had detectable tachyzoites in the brain for up to 37 days post-infection (while mice infected with WT parasites did not), and CNS transcriptional analyses at day 30 post-infection throughout the chronic phase of infection revealed inflammatory signatures consistent with acute infection in ?ROCY1 parasites compared to WT. Despite our inability to detect brain cysts in infected mice, both WT and ?ROCY1 knockout parasites reactivated after dexamethasone treatment with similar timing and magnitude for up to 5 months post infection, challenging the paradigm that long term parasite persistence in the CNS requires cyst formation. These data identify a new regulator of cyst formation in T. gondii that is both necessary and sufficient for cyst formation, and whose function relies on its conserved nucleic acid binding motif.
2023-08-11 | GSE211957 | GEO
Project description:The apicomplexan parasite Toxoplasma gondii can infect humans and almost all warm-blooded animals worldwide, poses significant threat to the public health and of veterinary importance. The acute infection was characterized by fast replication of tachyzoites inside the host cells. During this fast amplification process, the gene expression is highly regulated by series of regulator networks. The G1 phase is usually conserved across species and responsible for the preparation of materials for the next replication cell cycle, but seldom regulators were identified in this stage. Here, we functionally characterized the C/G1 phase expressed ApiAP2 transcription factor TgAP2XII-8 in T. gondii tachyzoite. Conditionally knockdown of TgAP2XII-8 leads to significant growth defects and asexual division disorder. Additionally, the parasite cell cycle progression was also disrupted after TgAP2XII-8 depletion, which is characterized by G1 phase arrest. RNA-seq and CUT&Tag experiments revealed that TgAP2XII-8 played as an activator for the ribosomal proteins expressed in G1 phase. Moreover, TgAP2XII-8 bind to a specific DNA motif ([T/C]GCATGCA), which is abundant and conserved in the intergenic region of several other apicomplexans, which may suggest a broad and conserved role for this ApiAP2 in the Phylum of Apicomplexa. These data provide important knowledge for the understanding of transcriptional regulation of parasite cell cycle in T. gondii.
2024-08-25 | GSE275112 | GEO