Project description:We report the expression of mRNA transcripts in X1 neoblast isolated by FACS followin amputation of the head (anterior wounding) and amputation of the pharyx and tail (posterior wounding) at 0 and 48 hours RNA-sequencing of pre-pharyngeal X1 cells from anterior and posterior facing wounds at 0 and 48 hrs after wounding in S. mediterranea
Project description:Investigation of whole genome gene expression level changes in nanos(RNAi) Schmidtea mediterranea, compared to control animals. nanos(RNAi) animals are devoid of germ cells The samples analyzed in this study are further described in Wang, Y., Stary, JM., Wilhelm, JE. and Newmark, PA. 2010. A eight chip study using total RNA recovered from asexual nanos(RNAi), asexual control, juvenile sexual nanos(RNAi), and juvenile sexual control animals. Each chip measures the expression level of 16,797 ESTs from S. mediterranea with 10 60-mer probe pairs (PM/MM) per gene, with two-fold technical redundancy.
