Project description:Our study makes use of zebrafish and has determined hepatic transcriptional changes after exposure to four single pharmaceuticals, a pharmaceutical mixture (MIX), and wastewater effluent (WWE) exposures. The pharmaceuticals chosen include acetaminophen, carbamazepine, gemfibrozil, and venlafaxine. We have performed chronic (6 week), low concentration (0.5 and 10 μgL-1 or 5 and 25% effluent) exposure of male and female fish and previously determined a range of effects (reproductive, histological, hormonal) after exposure to single compounds, a mixture of these compounds or wastewater effluent. Herein we determine by microarray the hepatic transcriptional responses of male and female zebrafish from the same exposures.
Project description:We tested the hypothesis that the behavioral response to selenium (Se) follows a hormetic dose response pattern, manifested through the functions of selenoproteins within the brain. We measured anxiety-related behaviors in zebrafish (Danio rerio) at deficient, control and supplemented levels of dietary Se, and measured the transcriptional response of selenoprotein genes important for neuroprotection. We also used a microarray approach to assess the transcriptomic response of the midbrain to Se. The behavioral response to Se was characterized by hormesis, and the direction, magnitude, and shape of the hormetic responses were dependent on both sex and zebrafish population. Transcription of selenoproteins within the midbrain also responded to Se in a similar hormetic dose-dependent manner, with sex and population influencing the trajectory of the responses. The hormetic behavioral response to Se may therefore be manifested through selenoproteins in the brain, but the influence is not direct. We performed a microarray analysis comparing the midbrain-specific transcriptome between male zebrafish from two populations (Pargana: P and Transgenic Mosaic 1: T) fed either a control, Se deficient, or Se supplemented diet (17 total samples: 9 fish per population, 3 fish per diet: missing 1 P control sample).
Project description:We tested the hypothesis that the behavioral response to selenium (Se) follows a hormetic dose response pattern, manifested through the functions of selenoproteins within the brain. We measured anxiety-related behaviors in zebrafish (Danio rerio) at deficient, control and supplemented levels of dietary Se, and measured the transcriptional response of selenoprotein genes important for neuroprotection. We also used a microarray approach to assess the transcriptomic response of the midbrain to Se. The behavioral response to Se was characterized by hormesis, and the direction, magnitude, and shape of the hormetic responses were dependent on both sex and zebrafish population. Transcription of selenoproteins within the midbrain also responded to Se in a similar hormetic dose-dependent manner, with sex and population influencing the trajectory of the responses. The hormetic behavioral response to Se may therefore be manifested through selenoproteins in the brain, but the influence is not direct.
Project description:We analyzed if genomic responses of adult zebrafish tissues can reproduce the mammalian known inflammatory process induced by acute endotoxin stress. Although the strength of the inflammatory process was influenced by tissue nature, gene regulation was well conserved across evolution and zebrafish genomic responses highly correlated with mammals’ inflammatory reactions after lipopolysaccharide stimulation.