Project description:Transcriptional profiling of Candida albicans comparing pterostilbene treated cells with untreated cells Wild type Candida albicans strain SC5314 was selected to carry out the expression profile microarray. Two-condition experiment, pterostilbene treated vs. untreated cells. Biological replicates: 3 control, 3 pterostilbene treated, independently grown and harvested. One replicate per array.
Project description:Transcriptional profiling of Candida albicans SC5314 comparing C. albicans grown in RPMI1640 or in RPMI1640 with 100ug/ml AAT. Goal was to determine the effects of AAT on global C. albicans gene expression.
Project description:Purpose: Cellular response of Candida albicans to Aureobasidin A at transcriptomic level. Method: The transcriptome of C. albicans SC5314 upon exposure to 1 µg/ml AbA (0.5X Minimum Inhibitory Concentration) for three hours was analyzed by RNA sequencing (RNA-Seq) on Illumina HiSeq 4000 instrument using the 2 X 150 bp chemistry. Result: Following STAR-alignment of the quality-checked sequence reads against SC5314 reference genome and DESeq2 analysis of the read counts data, we identified 85 differentially-expressed genes between the AbA-treated cells and DMSO-treated controls, with false discovery rate <0.001 and a log2 fold change ≥ 1. Among them, 42 were significantly up-regulated and 43 were significantly down-regulated in AbA-treated cells as compared to the controls. The analysis of biological processes and molecular functions using Gene Ontology (GO) Term Finder of the Candida Genome Database (CGD) showed down-regulation of genes associated with cellular lipid biosynthetic process, and up-regulation of genes associated with regulation of membrane lipid distribution and floppase activity. The genes associated with cell aggregation, biofilm formation and DNA packaging were also affected. Conclusion: In summary, the analysis of transcriptomic data in this study provides beneficial and critical information contributing to our understanding of the cellular response of C. albicans to AbA and its antifungal activity.
Project description:Homo sapiens fresh whole blood was infected with Candida albicans SC5314. RNA-pool of both species extracted at 0min (control), 15, 30, 60, 120, 240 min. Samples are rRNA depleted. Expression measurement of Homo sapiens genes.
