ABSTRACT: Effects on transcriptional regulation and lipid droplet characteristics in the liver of female juvenile pigs after early postnatal feed restriction and refeeding are dependent on birth weight
Project description:Low (U) and normal (N) birth weight female porcine offspring were used to study molecular and physiological changes in the liver before and after postnatal feed restriction (R, 50% of controls) and after subsequent refeeding period in comparison to non-restricted control animals (K). Overall, the following questions were addressed at the transcriptional, epigenomic and metabolic level: 1) Are there differences in the hepatic transcriptional profile between U and normal birth weight 2) Are these effects reflected on the metabolic level? 3) Could the possible birth weight-dependent effects be modified through feed restriction intervention? 4) Are these effects persistent and, moreover, can improvements with regard to lipid homeostasis be observed? Microarrays were used to study the effects of birth weight and/or feed restriction on the transcriptional level.
Project description:<p>Together with environmental factors, physiological maturity at birth is a major determinant for neonatal survival and postnatal development in mammalian species. Maturity at birth is the outcome of complex mechanisms of intra-uterine development and maturation during the end of gestation. In pig production, piglet preweaning mortality averages 20% of the litter and thus, maturity is a major welfare and economic concern. Here, we used both targeted and untargeted metabolomic approaches to provide a deeper understanding of the maturity in a model of lines of pigs divergently selected on residual feed intake (RFI), previously shown to have contrasted signs of maturity at birth. Analyses were conducted on plasma metabolome of piglets at birth and integrated with other phenotypic characteristics associated to maturity. We confirmed proline and myo-inositol, previously described for their association with delayed growth, as potential markers of maturity. Urea cycle and energy metabolism were found more regulated in piglets from high and low RFI lines, respectively, suggesting a better thermoregulation ability for the low RFI (with higher feed efficiency) piglets.</p>
Project description:Analysis of gene expression in the lungs of pigs from high and low litter birth weight groups (HBW and LBW) inoculated with swine influenza virus. The aim of the experiment is to determine whether litter birth weight has an effect on the innate immune response to infection in pigs, and whether differences in gene expression can be linked to epigenetic differences between the two birth weight groups.
Project description:Transcriptional profiling in liver of 16 pigs of 20 kg body weight from lines divergently selected for residual feed intake (RFI): low-RFI pigs (RFIneg), high-RFI pigs (RFIpl) .
Project description:Transcriptional profiling in skeletal muscle of 15 pigs of 20 kg body weight from lines divergently selected for residual feed intake (RFI): low-RFI pigs (RFIneg), high-RFI pigs (RFIpl) .
Project description:Transcriptional profiling in subcutaneous adipose tissue of 16 pigs of 20 kg body weight from lines divergently selected for residual feed intake (RFI): low-RFI pigs (RFIneg), high-RFI pigs (RFIpl) .
Project description:Analysis of gene expression in the lungs of pigs from high and low litter birth weight groups (HBW and LBW) inoculated with swine influenza virus. The aim of the experiment is to determine whether litter birth weight has an effect on the innate immune response to infection in pigs, and whether differences in gene expression can be linked to epigenetic differences between the two birth weight groups. A two condition experiment: lung tissue RNA from HBW and LBW SIV-infected pigs. 16 biological replicates for each condition, each hybridized with a reference to separate arrays. 32 arrays in total.
Project description:Transcriptional profiling in subcutaneous adipose tissue of 24 pigs of 115 kg body weight from lines divergently selected for residual feed intake (RFI): low-RFI pigs (RFIneg), high-RFI pigs (RFIpl) and high-RFI pigs restricted at RFIneg feeding level (RFIplR) to investigate the impact of feeding independently of selection.
2014-01-01 | GSE47771 | GEO
Project description:Gut microbiota of low birth weight pigs
Project description:Transcriptional profiling in liver of 24 pigs of 115 kg body weight from lines divergently selected for residual feed intake (RFI): low-RFI pigs fed ad libitum (RFIneg), high-RFI pigs fed ad libitum (RFIpl) and high-RFI pigs restricted at RFIneg feeding level (RFIplR) to investigate the impact of feeding independently of selection.
