Homo sapiens
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ABSTRACT:
PROVIDER: PRJNA187926 | ENA |
REPOSITORIES: ENA
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Project description:Hyperthermia (HT) is widely used to treat patients with various cancers. In general, HT elicits a wide spectrum of stress responses, such as induction of heat shock proteins, protein aggregation and cell death in mammalian cells. Although many biological processes are affected by HT, the overall responses to HT in mammalian cells remain unknown. The effects of heat stress at 41°C for 30 min (mild hyperthermia) on the gene expression in human oral squamous cell carcinoma HSC-3 cells were investigated using an Affymetrix GeneChip system. Human oral squamous cell carcinoma HSC-3 cells were treated with heat stress (41°C for 30 min), followed by incubation for 0, 1, or 3 h at 37°C. Non-treated cells served as control. Total RNA samples were prepared from the cells, and quality of the RNA was analyzed using a Bioanalyzer 2100. Gene expression was monitored by an Affymetrix GeneChip® system with a Human Genome U133-Plus 2.0 array. Sample preparation for array hybridization was carried out as described in the manufacturer's instructions.
2013-01-30 | E-GEOD-43862 | biostudies-arrayexpress
Project description:Hyperthermia (HT) is widely used to treat patients with various cancers. In general, HT elicits a wide spectrum of stress responses, such as induction of heat shock proteins, protein aggregation and cell death in mammalian cells. Although many biological processes are affected by HT, the overall responses to HT in mammalian cells remain unknown. The effects of heat stress at 41°C for 30 min (mild hyperthermia) on the gene expression in human oral squamous cell carcinoma HSC-3 cells were investigated using an Affymetrix GeneChip system.
2013-01-30 | GSE43862 | GEO
Project description:Identification of genes responsive to mild hyperthermia in human cervical squamous cell carcinoma HeLa cells
| PRJNA187084 | ENA
Project description:Hyperthermia is widely used to treat patients with various cancers. The 42.5ËC is well known as inflection point of hyperthermia and generally up to 42ËC of hyperthermia is used in clinical case to combine with other therapy. Here, the effects of heat stress at 42 or 44ËC for 90 min on the gene expression in HSC-3 human oral squamous carcinoma cells were investigated using an Affymetrix GeneChip system. The cells were treated with heat stress (42 or 44°C for 90 min) and followed by incubation for 0, 6, or 12 h at 37°C. The percentage of cell death was 5.0 ± 1.5 (mean ± SD) at 42°C for 12 h and 17.4 ± 0.6 at 44°C for 12 h. Of approximately 47,000 probe sets analyzed, many genes that were differentially expressed by a factor 2.0 or greater were identified in the cells treated with heat stress at 42 and 44°C. HSC-3 human oral squamous carcinoma cells were treated with heat stress (42 or 44°C for 90 min) and followed by incubation for 0, 6, or 12 h at 37°C. Non-treated cells were served as control. Total RNA samples were prepared from the cells. Gene expression was analyzed by an Affymetrix GeneChip® system with a Human Genome U133-plus 2.0 array for analysis of over 47,000 transcripts. Sample preparation for array hybridization was carried out as described in the manufacturerâs instructions.
2010-10-20 | E-GEOD-24783 | biostudies-arrayexpress
Project description:Identification of genes responsive to mild hyperthermia in human leukemia U937 cells
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Project description:The study aimed to resolve the mechanisms of protective actions of MMP-8 in oral tongue squamous cell carcinoma. The experiment compares the gene expression levels of control and MMP-8 overexpressing human oral tongue squamous cell carcinoma cells (HSC-3) in stationary and migrating phenotype.
2018-02-16 | GSE85435 | GEO
Project description:The effects of Candida albicans on the metastatic activity of oral squamous cell carcinoma was observed in vitro and in vivo. In the in vitro experimental setup HO-1-N-1 and HSC-2 human oral squamous cell carcinoma cell lines were treated with zymosan, heat-killed Candida albicans, heat-killed C. parapsilosis, live C. albicans and live C. parapsilosis. Whole transcriptomics was performed of the human tumor cells. In the in vivo experiment human HSC-2 tumor cells were injected to the tongue of mice. Whole transcriptomic analysis was performed of the human HSC-2 derived tumor cells comparing control tumor and oral candidiasis treated tumor.
2021-12-08 | GSE169278 | GEO
Project description:Hyperthermia is widely used to treat patients with various cancers. The 42.5˚C is well known as inflection point of hyperthermia and generally up to 42˚C of hyperthermia is used in clinical case to combine with other therapy. Here, the effects of heat stress at 42 or 44˚C for 90 min on the gene expression in HSC-3 human oral squamous carcinoma cells were investigated using an Affymetrix GeneChip system. The cells were treated with heat stress (42 or 44°C for 90 min) and followed by incubation for 0, 6, or 12 h at 37°C. The percentage of cell death was 5.0 ± 1.5 (mean ± SD) at 42°C for 12 h and 17.4 ± 0.6 at 44°C for 12 h. Of approximately 47,000 probe sets analyzed, many genes that were differentially expressed by a factor 2.0 or greater were identified in the cells treated with heat stress at 42 and 44°C.
2010-10-20 | GSE24783 | GEO
Project description:Identification of genes that are differentially regulated in fibroblasts derived from dysplastic oral mucosa and oral squamous cell carcinoma compared to fibroblasts derived from normal oral mucosa. Affymetrix microarrays were used to define differential gene expression. Populations of fibroblasts were isolated from human normal oral mucosa, oral dysplasia and oral squamous cell carcinoma, maintained in 3D collagen I biomatrices, RNA extracted and processed for Affymetrix arrays. Fibroblasts maintained as monolayers were also included as comparators.
2013-12-01 | E-GEOD-38517 | biostudies-arrayexpress
Project description:Identification of genes responsive to mild hyperthermia in human skin normal fibroblast Hs68 cells
| PRJNA170527 | ENA