Project description:AS1 and AS2 encode MYB related protein and AS2-domain containing protein, respectively and may regulate transcription. These genes are involved in the determination of axes of leaves of Arabidopsis thaliana. To know the gene regulation in the leaf development, expression profile among wild-type, as1 and as2 mutants and AS2 overexpression plants were compaired. shoot apices from as1-1, as2-1, AS2 overexpressing, and wild type embryos
Project description:AS1 and AS2 encode MYB related protein and AS2-domain containing protein, respectively and may regulate transcription. These genes are involved in the determination of axes of leaves of Arabidopsis thaliana. To know the gene regulation in the leaf development, expression profile among wild-type, as1 and as2 mutants and AS2 overexpression plants were compaired.
Project description:To investigate the function of long non-coding RNA (lncRNA) in ovarian endometriosis and explore its pathogenesis from a new prospective pathway, we conducted genome-wide microarray expression profiling.GO and KEGG analysis demonstrated that the differential mRNAs in ECvsEU and NOvsEU mostly focused on cell adhesion and biological adhesion pathways, nevertheless ECvsNO on molecular metabolism pathways. CHL1 was the most up-regulated mRNA in EC than EU, and the expression of its two antisense lncRNAs, CHL1-AS1 and CHL1-AS2 exhibited the same tendency. They were significantly correlated with each other through bioinformatic calculation.GSEA analysis revealed that CHL1-AS1 and CHL1-AS2 were conspicuously concerned with the same gene set. Validated by qRT-PCR, CHL1-AS2 was higher expressed in EC than EU. It was also up-regulated in NO, and the relative fold changes in EC/EU and NO/EU showed no statistical significance (P>0.05). Furthermore, the value of EC/NO was more closed to 1 when sample size was enlarged, presenting the near expression of CHL1-AS2 in EC and NO. Consequently, we supposed that CHL1, CHL1-AS1 and CHL1-AS2 may play an important part in ovarian endometriosis.
Project description:AS2 encode AS2-domain containing protein and may regulate transcription. AS2 is involved in the determination of axes of leaves of Arabidopsis thaliana. Type IB DNA topoisomerase (TOP1α) gene has genetic interaction with AS1 and AS2 and is involved in repression of leaf polarity genes. To know the gene regulation in the leaf development, expression profile among wild-type and as2 mutant treated with and without 10-hydroxyl substituted camptothecin (10H-CPT), a specific TOP1 inhibitor, were compared