Project description:Horsfieldia kingii is a member of Myristicaceae. The H. kingii chloroplast genome is found to be 155,655?bp in length and has a base composition of A (30.03%), G (19.52%), C (19.72%), and T (30.73%). The genome contained two short inverted repeat (IRa and IRb) regions (48,052?bp) which were separated by a large single copy (LSC) region (86,912?bp) and a small single copy (SSC) region (20,691?bp). The genome encodes 123 unique genes, including 85 protein-coding genes, 27 transfer RNA (tRNA) genes, and 8 ribosomal RNA (rRNA) genes. Further, complete chloroplast sequence of H. kingii was aligned together with other 2 species of Myristicaceae and other 5 basal angiosperms species which have reported the complete chloroplast sequence. This complete chloroplast genome will provide valuable information for the development of DNA markers for future species resource development and phylogenetic analysis of H. kingii.
Project description:Co-expression networks and gene regulatory networks (GRNs) are emerging as important tools for predicting the functional roles of individual genes at a system-wide scale. To enable network reconstructions we built a large-scale gene expression atlas comprised of 62,547 mRNAs, 17,862 non-modified proteins, and 6,227 phosphoproteins harboring 31,595 phosphorylation sites quantified across maize development. There was little edge conservation in co-expression and GRNs reconstructed using transcriptome versus proteome data yet networks from either data type were enriched in ontological categories and effective in predicting known regulatory relationships. This integrated gene expression atlas provides a valuable community resource. The networks should facilitate plant biology research and they provide a conceptual framework for future systems biology studies highlighting the importance of studying gene regulation at several levels.