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Expression data from Bifidobacterium animalis ssp. lactis strains exposed to hydrogen peroxide stress

ABSTRACT: Expression data from Bifidobacterium animalis ssp. lactis strains exposed to hydrogen peroxide stress

PROVIDER: PRJNA189708 | ENA |

REPOSITORIES: ENA

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Expression data from Bifidobacterium animalis ssp. lactis strains exposed to hydrogen peroxide stress

Project description:Stress survival tactics in bacteria utilize the up- and down-regulation of stress response genes. In bacteria that lack classical stress response genes for oxidative stress, other cellular systems can be used for cell survival. We used custom microarrays to study the regulation of genes in Bifidobacterium animalis ssp. lactis strains to oxidative stress to elucidate novel stress response mechanisms. Bifidobacterium cells were grown to late log phase then harvested and exposed to a sub-lethal level of hydrogen peroxide. Samples were taken at 5 and 20 mins for RNA extraction and hybridization on Affymetrix microarrays. Controls were prepared for each time point which recieved no hydrogen peroxide treatment.

2013-02-20 | E-GEOD-44382 | biostudies-arrayexpress

Expression data from Bifidobacterium animalis ssp. lactis strains exposed to hydrogen peroxide stress

2013-02-20 | GSE44382 | GEO

miRNAs expression in swine monocytes stimulated with Bifidobacterium animalis ssp. lactis Bb12

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Response of Bacillus pumilus to hydrogen peroxide

Project description:Reactive oxygen species such as hydrogen peroxide occur in all aerobically living organisms. Oxidative stress during fermentation can impair the fitness of the production host and the quality of the product. B. pumilus has been described as highly resistant to hydrogen peroxide. The response of exponentially growing B. pumilus cells to hydrogen peroxide was studied.

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Expression data from Caco-2 cells co-cultivated with B. animalis subsp. lactis BB-12

Project description:Although intestinal microbiota play a pivotal role in the development of host immune system this biological issue was not so far studied in great detail. In this study we examined immune response of Caco-2 enterocytes after incubation with common probiotic Bifidobacterium animalis subsp. lactis BB-12 for 4 hours. We used microarrays to inspect the global gene expression of Caco-2 cells upon co-culturing with B. animalis subsp. lactis BB-12 and several distinct immune-related genes up-regulated during this process. One time point (T4) and two controls (T0) were analysed. T0 represent differentiated Caco-2 cells cultivated for 3 weeks. T4 represents differentiated Caco-2 cells cultivated for 3 weeks plus consequent 4 hours of co-cultivation with B. animalis subsp. lactis BB-12. 3 technical replicates for T0-1, T0-2 or T4 were pooled to a single sample, RNA extracted and further used in gene expression experiments.

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