Project description:Hepatic transcriptome of sole fed low and high lipid dietary content in the early response to hypoxic stress. Characterization of the hepatic transcriptome of S. solea in order to assess the molecular mechanisms underlying the effects of dietary lipid content on hypoxia tolerance in common sole. S. solea individuals (8 month post-hatching) were fed for two month with commercial diets containing either 11% lipids or 20% lipids. Before hypoxia challenge stress, 38 individuals (19 from each dietary group) were randomly pooled in a single tank (1 m3) and left undisturbed and unfed for 48 h. The hypoxia challenge consisted then in a decrease of water oxygenation from 100 % to 10 % within one hour, followed by a slower descent at 2 % hr-1 to 4%. As fish lost their equilibrium, they were removed and sampled. Thirty six individuals (18 from each dietary group) were also sampled in normoxic conditions, resulting in 4 experimental groups: Normoxia low-lipid, Normoxia high-lipid, Hypoxia low-lipid, Hypoxia high-lipid. The experimental design was therefore a 2-way full factorial design between two juvenile dietary lipid contents and two oxygen concentration conditions.
Project description:A microarray platform for Solea solea was developed to study gene expression profiles of common sole developmental stages. Characterization of the transcriptome of S. solea, focusing on larval and juvenile stages. After transcriptome sequencing and annotation [TSA accession number GAAQ00000000], an oligo-DNA microarray for the detection of 12,836 unique transcripts was developed and applied to the study of gene expression profiles during larval-to-juvenile transition. For microarray experiments, pooled samples of 10–5 individuals (depending on larval age) were sampled and RNA extracted at 1, 4, 6, 11, 13, 18, 24 and 33 days post-hatching (dph). The number of biological replicates was four for each sampling point.
