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Elliptio complanata

ABSTRACT: Elliptio complanata Transcriptome or Gene expression

PROVIDER: PRJNA194430 | ENA |

REPOSITORIES: ENA

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Dataset's files

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			Action	DRS
	SRR5136461_1.fastq.gz	Fastqsanger.gz
	SRR5136461_2.fastq.gz	Fastqsanger.gz
	SRR5136462_1.fastq.gz	Fastqsanger.gz
	SRR5136462_2.fastq.gz	Fastqsanger.gz
	SRR5136463_1.fastq.gz	Fastqsanger.gz

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Impact of in vitro hormone treatments on the bibenzyl production of Radula complanata (Targeted metabolomics)

Project description:Bibenzyls are a specialized metabolite class found throughout the plant kingdom. One of the most prolific producers of bibenzyls are liverworts, specifically plants of the Radula genera. These plants possess an incredible diversity of bibenzyls, prenylated bibenzyls and a few (bis)bibenzyls, several of which have medicinal properties, including perrottetinene, an analog of tetrahydrocannabinol from cannabis. To provide insight into the bibenzyls’ biosynthesis in planta, exogenous phytohormones were applied to in vitro grown Radula complanata and bibenzyl metabolite production was monitored with targeted and untargeted metabolomics. The targeted metabolomic analysis of six prenylated bibenzyls revealed that production of these metabolites was largely reduced when plants were treated with abscisic acid (AA), salicylic acid (SA), 1-naphthaleneacetic acid (NAA) or 6-benzylaminopurine (BAP). The reduction of these metabolites in the BAP and NAA treatment suggests that prenylated bibenzyl production is negatively correlated with vegetative plant growth. The reduction of bibenzyls at low AA and SA concentrations and mild increase at higher AA and SA concentrations suggest that their production is regulated by these stress hormones. In addition, six other bibenzyl metabolites were tentatively identified from the untargeted analysis. These results provide insight into the influence of phytohormones on the bioactive bibenzyl content of R. complanata. Targeted metabolomics is reported in the current study <a href='https://www.ebi.ac.uk/metabolights/MTBLS4321' rel='noopener noreferrer' target='_blank'>MTBLS4321</a>.Untargeted metabolomics is reported in <a href='https://www.ebi.ac.uk/metabolights/MTBLS3563' rel='noopener noreferrer' target='_blank'>MTBLS3563</a>.

2024-01-10 | MTBLS4321 | MetaboLights

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