Project description:Jasmonates is inductively produced as a major plant hormone responsible for defense reactions in plants against both biotic and abiotic stresses, such as pathogen infection and mechanical wounding. We identified JA-inducible genes in the wild-type rice leaves 0 - 4 h after JA treatment using 44k microarray.
Project description:Jasmonates is inductively produced as a major plant hormone responsible for defense reactions in plants against both biotic and abiotic stresses, such as pathogen infection and mechanical wounding. We identified JA-inducible genes in the wild-type rice leaves 0 - 4 h after JA treatment using 44k microarray. Expression profiling in the wild-type rice leaves treated with jasmonic acid for 0.5, 1, 2, and 4 h was compared with that in the untreated wild-type rice leaves using two-color method with three biological replicates.
Project description:The plant hormone jasmonic acid (JA) has been known as a signal molecule that is induced by various stresses and mediates plant defense responses. Rice O. sativa inductively produces variety of defensive compounds upon abiotic and biotic stress conditions, such as wounding and insect attack. We identified wound-inducible genes by comparison with transcriptomes between wounded and untreated wild-type rice leaves.
Project description:5 leaves old rice plantlets were infected with Magnaporthe grisea spores and zero, two hours and twenty four houres after infection samples were collected
Project description:The plant hormone jasmonic acid (JA) has been known as a signal molecule that is induced by various stresses and mediates plant defense responses. Rice O. sativa inductively produces variety of defensive compounds upon abiotic and biotic stress conditions, such as wounding and insect attack. We identified wound-inducible genes by comparison with transcriptomes between wounded and untreated wild-type rice leaves. Expression profiling in wild-type rice leaves treated by wounding for 0.5, 1, 2 and 4 h was compared with that in untreated control using two-color method with two biological replicates.
Project description:The plant hormone jasmonic acid (JA) has been known as a signal molecule that is induced by various stresses and mediates plant defense responses. Rice O. sativa inductively produces variety of defensive compounds upon abiotic and biotic stress conditions, such as wounding and insect attack. The bHLH transcription factor RERJ1 has previously been identified as JA-inducible factor whose expression is also rapidly induced by wounding. We identified RERJ1-dependent and wound-inducible genes by comparison with transcriptomes of wound treated wild-type and a Tos17-rerj1 defective mutant rice. Expression profiling between rice leaves of wild-type and tos17-rerj1 mutant treated by wounding for 0, 0.5, 1 and 2 h was compared using two-color method with two biological replicates.
Project description:To understand the dynamics and global gene reprogramming in the early response to mechanical wounding in rice, the transcriptional response to mechanical injury was analyzed. A time-course experiment revealed the highly dynamic nature of the wound response in rice. Mechanical wounding triggered extensive gene expression reprogramming in the locally wounded leaf, affecting various physiological processes, including defense mechanisms and potentially tissue repair and regeneration. The rice response to mechanical wounding displayed both differences and similarities compared to the response to jasmonate treatment. These results highlight the importance of early JA signaling in response to mechanical stress in rice. This analysis provides an overview of the global transcriptional response to mechanical stress in rice, offering valuable insights for future studies on rice's response to injury, insect attack, and abiotic stresses.