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Talaromyces stipitatus

ABSTRACT: Non-pathogenic filamentous fungus closely related to the pathogenic fungus Penicillium marneffei

PROVIDER: PRJNA19561 | ENA |

REPOSITORIES: ENA

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			Action	DRS
		Other
	ABAS01.dat.gz	Other
	ABAS01.fasta.gz	Fasta.gz
	ABAS01.master.dat	Other

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Talaromyces marneffei PM1

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Unraveling the molecular basis of temperature-dependent genetic regulation in Penicillium marneffei

Project description:Comparative analysis of transcriptome of Penicillium marneffei PM1 grown at 25°C and 37°C Penicillium marneffei strain PM1 was pre-cultured at 25°C and 37°C for two weeks on Sabouraud's Dextrose Agar (SDA). Messenger RNAs were then isolated from one-week 25°C and 37°C cultures and sequenced with Illumina by BGI America. Two replicates.

2013-07-20 | E-GEOD-48898 | biostudies-arrayexpress

Talaromyces marneffei

Project description:Penicillium marneffei: Gene expression profiling during the dimorphic switch

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Talaromyces marneffei suppresses macrophage inflammation by regulating host alternative splicing

Project description:To investigate the function of NCOR2-013 in T. marneffei-infected THP-1 macrophages, we established NCOR2-013 overexpression THP-1 macrphages. We then performed gene expression profiling analysis using data obtained from RNA-seq of T. marneffei-infected NCOR2-013 overexpression THP-1 macrophages and T. marneffei-infected control cells.

2023-09-25 | GSE200512 | GEO

Penicillium marneffei: Gene expression profiling of hyphal and yeast cells and during conidiation

Project description:Penicillium marneffei (Talaromyces marneffei) is an opportunistic human pathogen that can grow in a multicellular hyphal form at 25°C or a unicellular fission yeast form at 37°C, and can switch between these two forms in response to temperature. The yeast form is found in infected individuals and represents the pathogenic phase. In response to specific environmental cues, the hyphal form can undergo asexual development (conidiation) the produce a differentiated multicellular structure called a conidiophore which produces dormant spores (conidia). Inhaled conidia initiate infection. To identify genes that are ‘phase or cell-state specific’ transcriptional profiling was performed with a custom microarray consisting of ~42% of the predicted P. marneffei genes and RNA from P. marneffei hyphal, yeast and conidiation cell types.

2013-11-21 | GSE51109 | GEO

Unraveling the molecular basis of temperature-dependent genetic regulation in Penicillium marneffei

Project description:Comparative analysis of transcriptome of Penicillium marneffei PM1 grown at 25°C and 37°C

2013-07-20 | GSE48898 | GEO

Penicillium marneffei: Gene expression profiling of hyphal and yeast cells and during conidiation

Project description:Penicillium marneffei (Talaromyces marneffei) is an opportunistic human pathogen that can grow in a multicellular hyphal form at 25M-BM-0C or a unicellular fission yeast form at 37M-BM-0C, and can switch between these two forms in response to temperature. The yeast form is found in infected individuals and represents the pathogenic phase. In response to specific environmental cues, the hyphal form can undergo asexual development (conidiation) the produce a differentiated multicellular structure called a conidiophore which produces dormant spores (conidia). Inhaled conidia initiate infection. To identify genes that are M-bM-^@M-^Xphase or cell-state specificM-bM-^@M-^Y transcriptional profiling was performed with a custom microarray consisting of ~42% of the predicted P. marneffei genes and RNA from P. marneffei hyphal, yeast and conidiation cell types. A custom microarray consisting of short, random genomic fragments from P. marneffei (~42% of the predicted genes) was generated using PCR products of the inserts from two independent DNA libraries constructed from genomic DNA of the P. marneffei type strain FRR2161. PCR products from previously cloned P. marneffei genes with known expression profiles were included as controls on the microarray. Total RNA from hyphal, yeast or conidiation cell types was prepared and used in pairwise combinations (conidiation versus hyphal cells, conidiation versus yeast cells and hyphal versus yeast cells) on the microarrays. Three biological replicate cultures were prepared for each experiment.

2013-11-21 | E-GEOD-51109 | biostudies-arrayexpress

RNA-seq of Talaromyces marneffei under mycelium and yeast conditions

Project description:Although many studies have focused on dimorphic switching, noncoding RNAs in Talaromyces marneffei have been neglected until now. rRNA depletion RNA-seq with or without RNase R treatment were performed in mycelium and yeast conditions of Talaromyces marneffei by to unveil the functions of circRNAs in dimorphic switching.

2023-05-08 | GSE231909 | GEO

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