Candida albicans
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA209387 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:The zinc cluster proteins are a family of transcription factors that are unique to the fungal kingdom. In the pathogenic yeast Candida albicans, zinc cluster transcription factors control the expression of virulence-associated traits and play key roles in the development of antifungal drug resistance. Gain-of-function mutations in several zinc cluster transcription factors, which result in constitutive overexpression of their target genes, are a frequent cause of azole resistance in clinical C. albicans isolates. We found that zinc cluster proteins can also be artificially activated by C-terminal fusion with the heterologous Gal4 activation domain. We used this strategy to create a comprehensive library of C. albicans strains expressing all 82 zinc cluster transcription factors of this fungus in a potentially hyperactive form. Screening of this library identified regulators of invasive filamentous growth and other phenotypes that are important during an infection. In addition, the approach uncovered several novel mediators of fluconazole resistance, including the multidrug resistance regulator Mrr2, which controls the expression of the major C. albicans multidrug efflux pump CDR1. Artificial activation therefore is a highly useful method to study the role of zinc cluster transcription factors in C. albicans and other fungi of medical, agricultural, and biotechnological importance.
2013-07-29 | GSE48259 | GEO
Project description:The zinc cluster proteins are a family of transcription factors that are unique to the fungal kingdom. In the pathogenic yeast Candida albicans, zinc cluster transcription factors control the expression of virulence-associated traits and play key roles in the development of antifungal drug resistance. Gain-of-function mutations in several zinc cluster transcription factors, which result in constitutive overexpression of their target genes, are a frequent cause of azole resistance in clinical C. albicans isolates. We found that zinc cluster proteins can also be artificially activated by C-terminal fusion with the heterologous Gal4 activation domain. We used this strategy to create a comprehensive library of C. albicans strains expressing all 82 zinc cluster transcription factors of this fungus in a potentially hyperactive form. Screening of this library identified regulators of invasive filamentous growth and other phenotypes that are important during an infection. In addition, the approach uncovered several novel mediators of fluconazole resistance, including the multidrug resistance regulator Mrr2, which controls the expression of the major C. albicans multidrug efflux pump CDR1. Artificial activation therefore is a highly useful method to study the role of zinc cluster transcription factors in C. albicans and other fungi of medical, agricultural, and biotechnological importance. In total, 15 samples are analysed: 3 replicates of 5 different strains. The 3 replicates of SC5314 are the wild type reference.
2013-07-29 | E-GEOD-48259 | biostudies-arrayexpress
Project description:Members of the Zinc Cluster Factor family alter virulence in Candida albicans
| PRJNA356057 | ENA
Project description:A family of secreted pathogenesis-related proteins in Candida albicans
| PRJNA139659 | ENA
Project description:The zinc cluster transcription factor Ahr1p directs Mcm1p regulation of Candida albicans adhesion
| PRJNA134433 | ENA
Project description:Global transcriptional profiling revealed that IL-17A induced artificial nutrient starvation conditions in Candida albicans, resulting in a downregulated target of rapamycin (TOR) signaling pathway and in increased autophagic responses and intracellular cAMP.
2012-01-18 | GSE35153 | GEO
Project description:The bacterium Lactobacillus rhamnosus antagonizes the fungus Candida albicans. The transcriptional response of C. albicans to the presence of L. rhamnosus in an in vitro infection model with and without antibiotic treatment was investigated using microarrays.
2022-04-27 | E-MTAB-11090 | biostudies-arrayexpress
Project description:Global transcriptional profiling revealed that IL-17A induced artificial nutrient starvation conditions in Candida albicans, resulting in a downregulated target of rapamycin (TOR) signaling pathway and in increased autophagic responses and intracellular cAMP. We used microarray to detail the global programme of gene expression underlying IL17A sensing by Candida albicans at different time points (T0_0h T1_4h, T2_24h) and identified distinct classes of up-regulated and down regulated genes.
2012-01-18 | E-GEOD-35153 | biostudies-arrayexpress
Project description:analysis Candida albicans NDT80 Family protein Rep1‘s interacting protein in glucose/galactose/glycerol
2022-10-18 | PXD037522 |