Project description:Genome-Scale draft model for Human Peripheral Blood Mononuclear Cells (PBMCs). A GEM for PBMCs was developed by applying the INIT
algorithm on Human Metabolic Reconstruction (HMR 2.0) as a template model. GEMs were contextualised/ constrained for different conditions using expression datasets. The gene/transcript expression data obtained from PBMCs of Type 1 Diabetes progressors, non-progressors, and healthy controls were employed to score each reaction of HMR 2.0. For further detail please refer to Electronic Supplementary Information of Sen et.al, Metabolic alterations in immune cells associate with progression to type 1 diabetes, Diabetologia, 15/01/2020, (https://doi.org/10.1007/s00125-020-05107-6).
Project description:Genome wide DNA methylation profiling of rheumatoid arthritis (RA) patients and healthy controls. The Illumina Infinium 450k Human DNA methylation Beadchip was used to obtain DNA methylation profiles in peripheral blood mononuclear cells (PBMCs). Samples included 25 RA patients and 18 healthy controls.
Project description:We profiled the expressioon of lncRNAs in the peripheral blood mononuclear cells (PBMCs) of PD patients and healthy controls. the enrichment of lncRNAs from PBMCs using Arraystar Human lncRNAs chip (Arraystar).
Project description:To understand the host response to this pathogen, we investigated the gene expression profiles of peripheral blood mononuclear cells (PBMCs) derived from SARS patients, and compared with healthy controls.
Project description:The objective of this experiment was to compare the transcriptomic profile (NanoString platform) of peripheral blood mononuclear cells (PBMCs) from COVID-19 patients with mild disease, and patients with severe COVID-19 with and without dexamethasone treatment, and healthy controls. We analyzed PBMCs from 4 mild COVID patients, 3 severe COVID patients,4 severe COVID patients treated with dexamethasone, and 5 healthy controls
Project description:Aberrant gene expression analysis between peripheral blood mononuclear cells (PBMCs) samples from healthy controls (HC) and patients with systemic lupus erythmatosus (SLE) were identified using Affymetrix gene arrays
Project description:Gene expression profiles of peripheral blood mononuclear cells (PBMCs) derived from patients with limited and diffuse SSc were analysed in comparison to gene expression profiles of PBMCs from sex and age matched healthy subjects.
Project description:To reveal the altered expression profiles of circular RNAs (circRNAs) in the peripheral blood mononuclear cells (PBMCs) of patients with retinopathy of prematurity (ROP), microarray analysis was conducted in the samples from ROP patients and controls.
Project description:Genome wide microRNA profiling in peripheral blood mononuclear cells from rheumatoid arthritis (RA) patients and healthy controls. The Affymetrix miRNA 4.0 chip was used to obtain RNA profiles in 28 RA patients and 18 healthy controls.
Project description:We performed single-cell RNA sequencing (scRNASeq) on cryopreserved peripheral blood mononuclear cells (PBMCs) of healthy controls and OTULIN haploinsufficient patients in order to clarify the perturbation, if any, of cellular phenotypes in various leukocyte subsets in vivo at basal state attributable to OTULIN haploinsufficiency.
