Project description:We report the application of next generation sequencing technology for high-throughput profiling of cytosine methylation in adult rat liver tissue.
Project description:We report the application of RNA sequencing with Next Generation Sequencing technology for high-throughput status of transcripts in wild-type and ubr1-deletion Beauveria bassiana cultured in germination media (GM).
Project description:We report the application of next generation sequencing technology for high-throughput profiling of cytosine methylation in adult rat liver tissue. To investigate the role of intragenic methylation, we generated the first high resolution methylation map of rat liver tissue and compared it with the proteome of the same rat tissue.
Project description:We report the application of next generation sequencing technology for high-throughput profiling of transcriptome in HCC murine cells treated by anti-PD-1 or IFN-α or anti-PD-1 combined with IFN-α
Project description:We report the application of RNA sequencing with Next Generation Sequencing technology for high-throughput status of transcripts in wild-type and Sur7-deletion Beauveria bassiana grown on nutrient-sufficient media (SDAY) or nutrient-limiting media (CDA), respectively
Project description:We report the application of next-generation sequencing technology for high-throughput profiling of H3K27ac and transcriptome analysis in pancreatic islets derived from C57Bl/6 mice fed a high-fat diet. We find genomic regions showing change in acetylation of histone H3K27 in response to long-term HFD feeding, which was significantly associated with differential gene expression. Furthermore, increased H3K27ac showed a distinctive genomic distribution surrounding proximal-promoter regions. This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cells under various environments.
Project description:We report the application of next-generation sequencing technology for high-throughput profiling of H3K27ac and transcriptome analysis in pancreatic islets derived from C57Bl/6 mice fed a high-fat diet. We find genomic regions showing change in acetylation of histone H3K27 in response to long-term (26 weeks) HFD feeding, which was significantly associated with differential gene expression. Furthermore, increased H3K27ac showed a distinctive genomic distribution surrounding proximal-promoter regions. This study provides a framework for the application of comprehensive chromatin profiling towards characterization of diverse mammalian cells under various environments.
Project description:High throughput transcriptomics using the TempO-Seq (TM) platform for dose response modelling of 3 different cell lines treated with the compound Benzophenone-4 as part of case study to explore the application of Next Generation Risk Assessment approaches for a sunscreen active ingredient. The data generated here was produced as one part of a toolbox of technologies to inform on bioactivity.