ABSTRACT: Expression data from dexamethasone treated mouse embryonic neural progenitor/stem cells isolated from wild type C57Bl/6 or caveolin-1 knockout mice
Project description:Expression data from dexamethasone treated mouse embryonic neural progenitor/stem cells isolated from wild type C57Bl/6 or caveolin-1 knockout mice
Project description:Neurosphere cultures prepared from E14.5 mouse cerebral cortex at passage 3 were treated for 4 hours with 100 nM dexamethasone We used microarrays to detail the global program of dexamethasone regulated gene expression in embryonic neural progenitor/stem cells. Cerebral cortex was isolated from E14.5 mouse fetuses and cultured as neurospheres for 3 passages prior to treatment with 100 nM dexamethasone or ethanol vehicle for 4 hours.
Project description:Neurosphere cultures prepared from E14.5 mouse cerebral cortex at passage 3 were treated for 4 hours with 100 nM dexamethasone We used microarrays to detail the global program of dexamethasone regulated gene expression in embryonic neural progenitor/stem cells
Project description:Expression data from dexamethasone treated mouse embryonic hypothalamic progenitor/stem cells isolated from wild type C57Bl/6 male or female mice
Project description:mRNA expression was compared in between wild type and caveolin-1 knockout livers mRNA expression was compared in between wild type and caveolin-1 knockout gonadal adipose tissue RNA was isolated from livers from male mice in at the light phase RNA was isolated from gonadal adipose tissues from male mice in at the light phase
Project description:mRNA expression was compared in between wild type and caveolin-1 knockout livers mRNA expression was compared in between wild type and caveolin-1 knockout gonadal adipose tissue
Project description:The goals of this study are to use assays for transposase accessible chromatin and dual-crosslinking immunoprecipitation, each followed by genome-wide sequencing (ATAC-seq and ChIP-seq, respectively) to measure chromatin accessibility and genomic GR binding patterns in vehicle-treated and dexamethasone-treated primary embryonic neural and progenitor stem cell cultures.
Project description:The goal of this study was to measure genome-wide expression in primary mouse neural stem/progenitor cell (NSPC) cultures to determine if SOX2 ablation alters the transcriptomic response which occurs following glucocorticoid receptor activation by the synthetic glucocorticoid, Dexamethasone. Neurosphere cultures of SOX2 knock out (KO) NSPCs and control non-deleted wild-type (WT) NSPCs (C57BL/6) derived from the fetal telencephalon were established at postnatal day zero (P0).
Project description:We used RNA-Seq to detail the global program of sexually dimorphic dexamethasone regulated gene expression in embryonic hypothalamic neural progenitor/stem cells.