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Deep sequencing reveals increased DNA methylation in chronic rat epilepsy

ABSTRACT: Deep sequencing reveals increased DNA methylation in chronic rat epilepsy

PROVIDER: PRJNA215949 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR955120.fastq.gz	Fastqsanger.gz
	SRR955121.fastq.gz	Fastqsanger.gz
	SRR955122.fastq.gz	Fastqsanger.gz
	SRR955123.fastq.gz	Fastqsanger.gz
	SRR955124.fastq.gz	Fastqsanger.gz

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Deep sequencing reveals increased DNA methylation in chronic rat epilepsy (mRNA-seq)

Project description:Explore DNA methylation in chronic epilepsy and its relationship to gene expression.

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Deep sequencing reveals increased DNA methylation in chronic rat epilepsy (MBD-seq)

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Deep sequencing reveals increased DNA methylation in chronic rat epilepsy (mRNA-seq)

Project description:Explore DNA methylation in chronic epilepsy and its relationship to gene expression. Examination of expression changes in pilocarpine-treated rats compared to controls and pilocarpine-treated rats on a ketogenic diet.

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Rat model of MTLE: Animals with epilepsy vs animals without epilepsy

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Metabolic Adaptation in Epilepsy: From Acute Response to Chronic Impairment

Project description:Epilepsy is characterized by hypersynchronous neuronal discharges, which are associated with an increased cerebral metabolic rate of oxygen and ATP demand. Uncontrolled seizure activity (status epilepticus) results in mitochondrial exhaustion and ATP depletion, which potentially generate energy mismatch and neuronal loss. Many cells can adapt to increased energy demand by increasing metabolic capacities. However, acute metabolic adaptation during epileptic activity and its relationship to chronic epilepsy remains poorly understood. We elicited seizure-like events (SLEs) in an in vitro model of status epilepticus for eight hours. Electrophysiological recording and tissue oxygen partial pressure recordings were performed. After eight hours of ongoing SLEs, we used proteomics-based kinetic modeling to evaluate changes in metabolic capacities. We compared our findings regarding acute metabolic adaptation to published proteomic and transcriptomic data from chronic epilepsy patients. Epileptic tissue acutely responded to uninterrupted SLEs by upregulating ATP production capacity. This was achieved by a coordinated increase in the abundance of proteins from the respiratory chain and oxidative phosphorylation system. In contrast, chronic epileptic tissue shows a 25-40% decrease in ATP production capacity. In summary, our study reveals that epilepsy leads to dynamic metabolic changes. Acute epileptic activity boosts ATP production, while chronic epilepsy reduces it significantly.

2024-09-12 | PXD055090 | Pride

Deep sequencing reveals increased DNA methylation in chronic rat epilepsy

Project description:This SuperSeries is composed of the SubSeries listed below.

2013-09-09 | GSE50080 | GEO

Genome-wide microRNA expression profiles of rat hippocampus in a chronic temporal lobe epilepsy model induced by amygdala stimulation

Project description:MicroRNAs (miRNAs) have been found to participate in the pathogenesis of several neurological diseases including epilepsy. To date, the expression and functions of miRNAs in chronic temporal lobe epilepsy (TLE), the most common type of refractory epilepsy in adults, have not been well characterized. Here, we adopted high-throughput sequencing to investigate miRNA expression profile in a chronic TLE model induced by amygdala stimulation

2017-02-09 | GSE52443 | GEO

Deep sequencing reveals increased DNA methylation in chronic rat epilepsy

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2013-09-09 | E-GEOD-50080 | biostudies-arrayexpress

Deep sequencing reveals increased DNA methylation in chronic rat epilepsy.

Project description:Epilepsy is a frequent neurological disorder, although onset and progression of seizures remain difficult to predict in affected patients, irrespective of their epileptogenic condition. Previous studies in animal models as well as human epileptic brain tissue revealed a remarkably diverse pattern of gene expression implicating epigenetic changes to contribute to disease progression. Here we mapped for the first time global DNA methylation patterns in chronic epileptic rats and controls. Using methyl-CpG capture associated with massive parallel sequencing (Methyl-Seq) we report the genomic methylation signature of the chronic epileptic state. We observed a predominant increase, rather than loss of DNA methylation in chronic rat epilepsy. Aberrant methylation patterns were inversely correlated with gene expression changes using mRNA sequencing from same animals and tissue specimens. Administration of a ketogenic, high-fat, low-carbohydrate diet attenuated seizure progression and ameliorated DNA methylation mediated changes in gene expression. This is the first report of unsupervised clustering of an epigenetic mark being used in epilepsy research to separate epileptic from non-epileptic animals as well as from animals receiving anti-convulsive dietary treatment. We further discuss the potential impact of epigenetic changes as a pathogenic mechanism of epileptogenesis.

| S-EPMC3825532 | biostudies-literature

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