Project description:Fructose has recently been observed to affect brain metabolism and cognitive function in adults. However, possible fetal programming of brain metabolism induced by gestational fructose has not been examined. We evaluated mitochondrial function in the brain of aging (15 month) male offspring of Fischer F344 rat dams fed either a high-fructose diet (50% energy from fructose) or a matched control diet during early life (gestation and lactation). We used microarrays to detail changes in global programme of gene expression in offspring brains from rats subjected to either a control diet or a high fructose diet during gestation and lactation
Project description:In order to establish a rat embryonic stem cell transcriptome, mRNA from rESC cell line DAc8, the first male germline competent rat ESC line to be described and the first to be used to generate a knockout rat model was characterized using RNA sequencing (RNA-seq) analysis.
