Project description:In order to facilitate understanding of pigment cell biology, we developed a method to concomitantly purify melanocytes, iridophores, and retinal pigmented epithelium from zebrafish, and analyzed their transcriptomes. Comparing expression data from these cell types and whole embryos allowed us to reveal gene expression co-enrichment in melanocytes and retinal pigmented epithelium, as well as in melanocytes and iridophores. We found 214 genes co-enriched in melanocytes and retinal pigmented epithelium, indicating the shared functions of melanin-producing cells. We found 62 genes significantly co-enriched in melanocytes and iridophores, illustrative of their shared developmental origins from the neural crest. This is also the first analysis of the iridophore transcriptome. Gene expression analysis for iridophores revealed extensive enrichment of specific enzymes to coordinate production of their guanine-based reflective pigment. We speculate the coordinated upregulation of specific enzymes from several metabolic pathways recycles the rate-limiting substrate for purine synthesis, phosphoribosyl pyrophosphate, thus constituting a guanine cycle. The purification procedure and expression analysis described here, along with the accompanying transcriptome-wide expression data, provide the first mRNA sequencing data for multiple purified zebrafish pigment cell types, and will be a useful resource for further studies of pigment cell biology. mRNA profiles of zebrafish pigment cells were generated using Illumina GAIIX sequencing

2013-04-26 | E-GEOD-46387 | biostudies-arrayexpress

Danio rerio

Project description:Expression profiling of the retina of pde6c, a zebrafish model of retinal degeneration

| PRJNA394760 | ENA

Danio rerio

Project description:Yap and Taz regulate retinal pigment epithelial cell fate

| PRJNA291981 | ENA

Danio rerio

Project description:Gene Expression Analysis of Zebrafish Melanocytes, Iridophores, and Retinal Pigmented Epithelium Reveals Indicators of Biological Function and Developmental Origin

| PRJNA198908 | ENA

Danio rerio

Project description:Zebrafish 24hpf mutant sk8 gene expression

| PRJNA111131 | ENA

Danio rerio

Project description:Expression profiling of zebrafish sox9 mutants reveals that Sox9 is required for retinal differentiation

| PRJNA110159 | ENA

TGFβ signaling in retinal tissue repair [Danio Rerio]

Project description:In mammals, retinal damage is followed by Müller glia cell activation and proliferation. While retinal gliosis persists in adult mammals after an insult or disease, some vertebrates, including zebrafish, have the capacity to regenerate. We believe we are the first group to show that gliosis is a fibrotic-like process in mammals’ eyes caused by differential activation of canonical and non-canonical TGFβ signaling pathways.

2019-11-03 | GSE132141 | GEO

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