Escherichia coli
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA218553 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:This SuperSeries is composed of the following subset Series: GSE12877: Transcriptional profiling of Escherichia coli after addition of CO-RMs to aerobically growing cells GSE12878: Transcriptional profiling of Escherichia coli after addition of CO-RMs to anaerobically growing cells Refer to individual Series
2010-06-24 | E-GEOD-13048 | biostudies-arrayexpress
Project description:Transcriptional profiling of Escherichia coli after addition of CO-RMs to aerobically growing cells
| PRJNA114165 | ENA
Project description:Transcriptional profiling of Escherichia coli after addition of CORM-401 to aerobically and anaerobically growing cells
| PRJNA292077 | ENA
Project description:Transcriptional profiling of Escherichia coli after addition of CO-RMs to anaerobically growing cells
| PRJNA114167 | ENA
Project description:The transcriptome of Escherichia coli K-12 has been widely studied over a variety of conditions for the past decade while such studies involving E. coli O157:H7, its pathogenic cousin, are just now being conducted. To better understand the impact of an anaerobic environment on E. coli O157:H7, global transcript levels of strain EDL933 cells grown aerobically were compared to cells grown anaerobically using microarrays.
2010-12-01 | GSE13301 | GEO
Project description:Transcriptional profiling of Escherichia coli after addition of CORM-3 and iCORM-3 to aerobically and anaerobically growing cells
| PRJNA175034 | ENA
Project description:We recently reported that carbon monoxide (CO) has bactericidal activity. To understand its mode of action we analysed the gene expression changes occurring when Escherichia coli, grown aerobically and anaerobically, is treated with the carbon monoxide releasing molecule, CORM-2. The E. coli microarray analysis shows that E. coli CORM-2 response is multifaceted with a high number of differentially regulated genes spread through several functional categories, namely genes involved in inorganic ion transport and metabolism, regulators, and genes implicated in posttranslational modification, such as chaperones. CORM-2 has higher impact in E. coli cells grown anaerobically, as judged by the existence of repressed genes belonging to eight functional classes which are absent in aerobically CORM-2 treated cells. In spite of the relatively stable nature of the CO molecule, our results show that CO is able to trigger a significant alteration in the transcriptome of E. coli which necessarily has effects in several key metabolic pathways.
2008-12-17 | GSE13982 | GEO
Project description:We recently reported that carbon monoxide (CO) has bactericidal activity. To understand its mode of action we analysed the gene expression changes occurring when Escherichia coli, grown aerobically and anaerobically, is treated with the carbon monoxide releasing molecule, CORM-2. The E. coli microarray analysis shows that E. coli CORM-2 response is multifaceted with a high number of differentially regulated genes spread through several functional categories, namely genes involved in inorganic ion transport and metabolism, regulators, and genes implicated in posttranslational modification, such as chaperones. CORM-2 has higher impact in E. coli cells grown anaerobically, as judged by the existence of repressed genes belonging to eight functional classes which are absent in aerobically CORM-2 treated cells. In spite of the relatively stable nature of the CO molecule, our results show that CO is able to trigger a significant alteration in the transcriptome of E. coli which necessarily has effects in several key metabolic pathways. Experiment Overall Design: Total RNA from two independent cultures of cells grown either aerobically or anaerobically and treated with 250 ?M CORM-2 during 15 min or left untreated were processed for use in the Affymetrix GeneChip E. coli Genome 2.0 Arrays, according to the manufacturer's instructions. Arrays were scanned in an Affymetrix GeneChip scanner 2500 and analysed first with Affymetrix MAS 5.0 software to obtain Absent/Present calls and to assure that all quality parameters were within the recommended range. Hybridization, scanning and detection procedures were done at the Genomics Unit of Instituto Gulbenkian de Ciencia (Portugal).
2009-01-13 | E-GEOD-13982 | biostudies-arrayexpress
Project description:The transcriptome of Escherichia coli K-12 has been widely studied over a variety of conditions for the past decade while such studies involving E. coli O157:H7, its pathogenic cousin, are just now being conducted. To better understand the impact of an anaerobic environment on E. coli O157:H7, global transcript levels of strain EDL933 cells grown aerobically were compared to cells grown anaerobically using microarrays. Eight independent RNA samples from aerobic cultures were paired with eight independent RNA samples from anaerobic cultures for hybridization to eight two-color microarrays. For four arrays, the control RNA sample was labeled with Cy3 dye and the experimental RNA sample was labeled with Cy5 dye, the dyes were reversed for the other six arrays to overcome dye bias.
2010-12-01 | E-GEOD-13301 | biostudies-arrayexpress
Project description:Transcriptional profiling of E. coli after addition of peroxynitrite or hydrogen peroxide to aerobically growing cells
| PRJNA120413 | ENA