Gut metagenome
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA227053 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Fecal bacterial amplicons of dogs fed graded levels of dietary resistant starch
| PRJNA227052 | ENA
Project description:Resistant starches (RS), fed as high amylose maize starch (HAMS) or butyrylated HAMS (HAMSB), oppose dietary protein-induced colonocyte DNA damage in rats. In this study, rats were fed diets high in fat (19%) and protein (20%) with different forms of digestible starch (low amylose maize starch (LAMS) or low amylose whole wheat (LAW)) or RS (HAMS, HAMSB, or a whole high amylose wheat (HAW) generated by RNA interference (RNAi)) for 11 wk. A control diet contained 7% fat, 13% protein and LAMS. The aim of this study was to detect changes in the expression of DNA damage and repair genes in response to the above dietary treatments.
2012-12-01 | GSE32312 | GEO
Project description:Dietary methionine restriction represses growth and improves therapeutic responses in several pre-clinical settings. However, how this dietary intervention impacts cancer progression in the context of the immune system is unknown. Here we analyzed the CD45+ immune cells from the small intestine of control (CTRL) diet or methionine-restricted (MR) diet fed tumor-free C57BL/6J donor mice and tumor-bearing Apc <min+/-> recipient mice transplanated with feces from these diet-fed tumor-free C57BL/6J mice by scRNA-seq. Our analysis indicate that fecal microbes from methionine-restricted tumor-free C57BL/6J mice are sufficient to represss T cell activation in the small intestine of Apc <min+/-> mice.
2023-06-14 | GSE181220 | GEO
Project description:Fecal bacterial amplicons of overweight and obese client-owned dogs fed cooked bean powders. Genome sequencing
| PRJNA257299 | ENA
Project description:This study in rats was designed to investigate whether whole rhye (WR) can influence the metabolism of n-3 and n-6 long-chain fatty acids (LCFA) and gut microbiota composition. For 12 weeks, rats were fed a diet containing either 50% WR or 50% refined rye (RR). Total bacterial DNA was extracted from fecal and cecal samples (n=5 per group). 16S PCR amplification was performed to assess the microbial diversity at the family level using the HuGChip. Amplified DNA was purified and labelled with either Cy3 or Cy5 dye and hybridized on the microarray. A 15 chip study was realized, each corresponding to hybridization with 250ng of labelled 16S rRNA gene amplicons from either mice fecal and cecal samples. Each probe (4441) was synthetized in three replicates.
2015-08-06 | E-GEOD-71726 | biostudies-arrayexpress
Project description:A common-garden experiment was carried out to compare two genetically distinct strains of Atlantic salmon (Salmo salar) fed diets formulated with either high (CHO) or low (NoCHO) carbohydrate (starch). Twenty salmon from either a commercial farmed strain or a land-locked population were placed in two tanks (10 fish of each population in each tank) and fed either CHO or NoCHO feeds for 32 days. At the end of the experimental period fish were fasted for 8 h, euthanized and samples of blood and liver collected. Both diet and population had an effect on circulating glucose levels with land-locked salmon showing hypoglycaemia and dietary starch increasing this parameter. In contrast, land-locked salmon showed increased plasma triacylglycerol levels regardless of dietary treatment. This enhanced ability to metabolise dietary starch in land-locked compared to farmed salmon stock was also reflected at a molecular (gene) level as most of the metabolic pathways evaluated in the present study were mainly affected by the factor population rather than by diet. In particular, lower expression of genes for mitochondrial metabolism in land-locked salmon reflects drastic differences in energy metabolism between the populations. The liver transcriptome analysis highlighted some new gene candidates such as elovl6 to evaluate in future studies assessing the capacity of salmonids to cope with feeds containing higher levels of dietary starch.
2018-12-31 | E-MTAB-6188 | biostudies-arrayexpress
Project description:Fecal microbiome of healthy adult dogs after an abrupt dietary change
| PRJNA846742 | ENA
Project description:Impact of dietary fiber and starch levels in shaping cecal microbiota revealed in rabbits
| PRJNA184687 | ENA
Project description:Resistant starches (RS), fed as high amylose maize starch (HAMS) or butyrylated HAMS (HAMSB), oppose dietary protein-induced colonocyte DNA damage in rats. In this study, rats were fed diets high in fat (19%) and protein (20%) with different forms of digestible starch (low amylose maize starch (LAMS) or low amylose whole wheat (LAW)) or RS (HAMS, HAMSB, or a whole high amylose wheat (HAW) generated by RNA interference (RNAi)) for 11 wk. A control diet contained 7% fat, 13% protein and LAMS. The aim of this study was to detect changes in the expression of DNA damage and repair genes in response to the above dietary treatments. Distal colon tissues from Sprague Dawley rats fed a variety of diets (see summary) were removed from RNAlater stabilisation reagent (Sigma, Australia) they had been stored in,, placed in 1 ml of TRIzol® Reagent (Invitrogen, Australia) and homogenised usingbeads (mix of 2.5 mm glass and 0.1 - 1.0 mm diameter silicon-zirconian beads) in a MiniBeadbeater-8 (BioSpec Products Inc., USA). Total RNA was extracted (using TRIzol® Reagent manufacturer's instructions) and further purified using RNAeasy mini spin columns (QIAGEN, Australia) with a DNase on-column digestion as per manufacturer's instructions.RNA integrity was checked using a Bioanalyzer 2100 (Agilent Technologies, USA) andquantified using a NanoDrop® ND-1000 Spectrophotometer (Thermo Fisher Scientific, USA). RNA samples with insufficinet quality and quantity were not assayed. The numbers of rats arrayed from each diet were as follows: CON, 5;LAMS, 7; HAMS, 8; HAMSB, 9; LAW, 6; HAW , 8. This gives a total of 43 arrays.
2012-12-01 | E-GEOD-32312 | biostudies-arrayexpress