Project description:To investigate the role of PDEF in prostate cancer metastasis, we employed microarray analysis of PC3 cells ectopically expressing a pBABE vector control (PC3-SCR) or PDEF-containing vector (PC3-PDEF).

2020-01-24 | GSE108641 | GEO

Homo sapiens

Project description:Gene expression analysis of osteosarcoma tumor-initiating cells (High) vs bulk tumor cells (Low)

| PRJNA148675 | ENA

E-GEOD-52127

Project description:Brain tumor initiating cell response to microglia

2025-01-24 | E-GEOD-52127 | ExpressionAtlas

Expression data from prostate cancer and osteoblast co-cultures

Project description:Dynamic interaction between prostate cancer and the bone microenvironment is a major contributor to metastasis of prostate cancer to bone. In this study we utilized an in-vitro co-culture model of PC3 prostate cancer cells and osteoblasts followed by microarray based gene expression profiling to identify previously unrecognized prostate cancer-bone microenvironment interactions. Factors secreted by PC3 cells resulted in the up-regulation of many genes in osteoblasts associated with bone metabolism and cancer metastasis, including Mmp13, Il-6 and Tgfb2, and down-regulation of Wnt inhibitor Sost. To determine whether altered Sost expression in the bone microenvironment has an effect on prostate cancer metastasis, we co-cultured PC3 cells with Sost knockout (SostKO) osteoblasts and wildtype (WT) osteoblasts and identified several genes differentially regulated between PC3-SostKO osteoblast co-cultures and PC3-WT osteoblast co-cultures. Co-culturing PC3 cells with WT osteoblasts up-regulated cancer-associated long noncoding RNA (lncRNA) MALAT1 in PC3 cells. MALAT1 expression was further enhanced when PC3 cells were co-cultured with SostKO osteoblasts and treatment with recombinant Sost down-regulated MALAT1 expression in these cells. Our results suggest that reduced Sost expression in the tumor microenvironment may promote bone metastasis by up-regulating MALAT1 in prostate cancer.

2015-09-30 | GSE73044 | GEO

E-GEOD-58078

Project description:Microarray analysis of WT and Tfap4-KO CD8 T cells during early activation

2015-07-29 | E-GEOD-58078 | ExpressionAtlas

Homo sapiens

Project description:Genes regulated by GRK3 in PC3 prostate cancer cells

| PRJNA151745 | ENA