Project description:miRNA expression analyses of Tif1b-deficient hematopoietic stem cells (HSCs). Tif1b loss leds to rapid depletion of HSCs. Expression analyses provide insight into the role of Tif1b in the maitenance of HSC.
Project description:miRNA expression analyses of Tif1b-deficient hematopoietic stem cells (HSCs). Tif1b loss leds to rapid depletion of HSCs. Expression analyses provide insight into the role of Tif1b in the maitenance of HSC. HSCs (c-Kit+ Sca1+ Lineage- ) are sorted from adult bone marrow and are subjected into microarray analyses.
Project description:Gene expression analyses of Tif1b-deficient hematopoietic stem cells (HSCs). Tif1b loss leads to rapid depletion of HSCs. Expression analyses provide insight into the role of Tif1b in the maitenance of HSC.
Project description:Gene expression analyses of Tif1b-deficient hematopoietic stem cells (HSCs). Tif1b loss leads to rapid depletion of HSCs. Expression analyses provide insight into the role of Tif1b in the maitenance of HSC. HSCs (c-Kit+ Sca1+ Lineage- ) are sorted from fetal liver or adult bone marrow and are subjected into microarray analyses.
Project description:The transcriptome of Ctrl and Vitamin A-deficient longterm hematopoietic stem cells (LT-HSC) and multipotant progenitors (MPP3/4) was assessed by RNAseq.
Project description:A transcriptome study in mouse hematopoietic stem cells was performed using a sensitive SAGE method, in an attempt to detect medium and low abundant transcripts expressed in these cells. Among a total of 31,380 unique transcript, 17,326 (55%) known genes were detected, 14,054 (45%) low-copy transcripts that have no matches to currently known genes. 3,899 (23%) were alternatively spliced transcripts of the known genes and 3,754 (22%) represent anti-sense transcripts from known genes.
Project description:A transcriptome study in mouse hematopoietic stem cells was performed using a sensitive SAGE method, in an attempt to detect medium and low abundant transcripts expressed in these cells. Among a total of 31,380 unique transcript, 17,326 (55%) known genes were detected, 14,054 (45%) low-copy transcripts that have no matches to currently known genes. 3,899 (23%) were alternatively spliced transcripts of the known genes and 3,754 (22%) represent anti-sense transcripts from known genes. Mouse hematopoietic stem cells were purified from bone marrow cells using negative and positive selection with a Magnetic-Activated Cell Sorter (MACS). total RNA and mRNA were purified from the purified cells using Trizol reagent and magnetic oligo dT beads. Double strand cDNAs were synthesized using a cDNA synthesis kit and anchored oligo dT primers. After NlaIII digestion, 3’ cDNAs were isolated and amplified through 16-cycle PCR. SAGE tags were released from the 3’ cDNA after linker ligation. Ditags were formed, concatemerized and cloned into a pZERO vector. Sequencing reactions were performed with the ET sequencing terminator kit. Sequences were collected using a Megabase 1000 sequencer. SAGE tag sequences were extracted using SAGE 2000 software.
Project description:Gene expression analyses of Tif1b, Hp1a or Hp1g knockdown hematopoietic progenitors. Growth of hematopoietic stem cells (HSCs) are significantly impaired upon knockdown of Hp1a and Hp1g. Results provide insight into the role of these factors in hematopoiesis. HSCs (CD34- c-Kit+ Sca1+ Lineage- ) were transduced with lentivirus expressing shRNA against Tif1b, Hp1a or Hp1g, and cultured for 14 days. Then, GFP+ c-Kit+ Lineage- hematopoietic progenitors were sorted and subjected into microarray analysis
