Project description:Progesterone receptor (PR) is expressed from a single gene as two isoforms, PRA and PRB. In normal breast human tissue, PRA and PRB are expressed in equimolar ratios, but isoform ratio is altered during malignant progression, usually leading to high PRA:PRB ratios. We took advantage of a transgenic mouse model where PRA isoform is predominant (PRA transgenics) and identified the key transcriptional events and associated pathways underlying the preneoplastic phenotype in mammary glands of PRA transgenics as compared with normal wild-type littermates.
Project description:We isolated mir-284 from a genetic screen for age-dependent axon degeneration in Drosophila melanogaster. mir-284 levels show a steep decline with aging. Therefore we embarked on a transcriptome-wide analysis after mir-284 depletion in aging flies.