Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Drosophila melanogaster

ABSTRACT: small RNA deep-sequencing with oxidation/β-elimination with age in Drosophila

PROVIDER: PRJNA230556 | ENA |

REPOSITORIES: ENA

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	SRR1042403.fastq.gz	Fastqsanger.gz
	SRR1042404.fastq.gz	Fastqsanger.gz
	SRR1042405.fastq.gz	Fastqsanger.gz
	SRR1042406.fastq.gz	Fastqsanger.gz

Items per page:

1 - 4 of 4

Similar Datasets

small RNA deep-sequencing with oxidation/β-elimination with age in Drosophila

Project description:Drosophila miRNAs show distinct change in isoform distribution pattern with age. Some miRNAs show accumulation of the short isoforms, while other miRNAs show the accumulation of the long isoforms with age. The increase of the long isoforms of some miRNAs reflects increased 2'-O-methylated miRNA isoforms with age. This raised a question on whether additional miRNAs show increased 2'-O-methylated miRNA isoforms with age. To investigate change in 2'-O-methylated small RNA population with age, we performed small RNA deep-sequencing of oxidized or non-oxidized small RNAs at 3d and 30d in Drosophila. This analysis revealed change in global pattern of 2'-O-methylated miRNA isoforms with age. 3d and 30d wild-type male flies were collected. Total RNA was prepared with Trizol reagent, followed by oxidation/β-elimination assay or the control treatment (no-oxidation). Small RNA libraries were prepared using Illumina's TruSeq small RNA sample preparation kit (#RS-200-0012, Illumina, Inc. San Diego, CA), following the manufacturer's protocol. The libraries were multiplexed and sequenced on HiSeq2000 platform (Illumina).

2014-01-01 | E-GEOD-52960 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Ago1 vs Ago2-IP small RNA deep-sequencing with age in Drosophila

| PRJNA230555 | ENA

Analysis of the 3’-end of piRNAs in the COLO 205 cell line through sodium periodate (NaIO4) /β-Elimination treatment and small RNA-Seq

Project description:Using a chemical treatment, the sodium periodate (NaIO4) /β-Elimination oxidation, and then performing deep-sequencing of small RNAs with NGS technologies, we evaluated the methylation status of the piRNAs population in the metastatic COLO 205 cell line.

2020-08-06 | E-MTAB-8115 | biostudies-arrayexpress

Drosophila melanogaster

Project description:Profling of the Drosophila melanogaster neuronal microRNA transcriptome by small RNA deep sequencing

| PRJNA247725 | ENA

Drosophila melanogaster

Project description:Drosophila melanogaster small RNA uridylase

| PRJNA280626 | ENA

Drosophila melanogaster

Project description:Small RNA Seq in Drosophila testes

| PRJNA439350 | ENA

Deep peptidomics analysis of Drosophila melanogaster

Project description:A deep peptidomics workflow was used to identify alternative proteins in Drosophila melanogaster samples.

2022-01-07 | MSV000088656 | MassIVE

Diurnal small RNA expression and post-transcriptional regulation in young and old Drosophila melanogaster heads

Project description:MicroRNAs are a class of small (~22nt) endogenous RNAs that regulate target transcript expression post-transcriptionally. Previous studies characterized age-related changes in diurnal transcript expression but it is not understood how these changes are regulated, and whether they may be attributed in part to changes in microRNA expression or activity with age. Diurnal small RNA expression changes with age were not previously studied. To interrogate changes in small RNA expression with age, we collected young (5 day) and old (55 day) Drosophila melanogaster around-the-clock and performed deep sequencing on size-selected RNA from whole heads. We find several microRNAs with changes in rhythmicity after aging, and we investigate microRNAs which are differentially expressed with age. We find that predicted targets of differentially expressed microRNAs have RNA-binding and transcription factor activity. We use a previously published method to identify mRNA transcripts which show evidence of microRNA targeting that is altered after aging, and find several that are involved in muscle development and maintenance. Finally, we identify novel microRNAs using the random-forest-based method miRWoods, which surprisingly also discovered transfer RNA-derived fragments.

2022-08-31 | GSE210559 | GEO

Drosophila melanogaster

Project description:modENCODE RNA-Seq of Drosophila Kc167 small RNAs

| PRJNA119891 | ENA

Drosophila melanogaster

Project description:Drosophila melanogaster small RNA sequence from 14 libraries

| PRJNA106137 | ENA