Project description:Gene-specific two-way ANOVA analysis for identification of candidate genes for processing quality, seed development, and interaction (quality x development) Gene expression data of two good chapatti making varieties, C306 and Lok1 and two poor chapatti making varieties, sonalika and WH291 at three seed development stages (7, 14, and 28 days after anthesis) were analyzed using gene-specific two-way ANOVA to parition varition in gene expression due to quality, seed development, and their interaction (quality x development).
Project description:Gene-specific two-way ANOVA analysis for identification of candidate genes for processing quality, seed development, and interaction (quality x development)
Project description:To better understand the regulatory mechanisms of water stress response in wheat, the transcript profiles in roots of two wheat genotypes, namely, drought tolerant 'Luohan No.2' (LH) and drought susceptible 'Chinese Spring' (CS) under water-stress were comparatively analyzed by using the Affymetrix wheat GeneChip®. A total of 3831 transcripts displayed 2-fold or more expression changes, 1593 transcripts were induced compared with 2238 transcripts were repressed, in LH under water-stress; Relatively fewer transcripts were drought responsive in CS, 1404 transcripts were induced and 1493 were repressed. Comparatively, 569 transcripts were commonly induced and 424 transcripts commonly repressed in LH and CS under water-stress. 689 transcripts (757 probe sets) identified from LH and 537 transcripts (575 probe sets) from CS were annotated and classified into 10 functional categories, and 74 transcripts derived from 80 probe sets displayed the change ratios no less than 16 in LH or CS. Several kinds of candidate genes were differentially expressed between the LH and CS, which could be responsible for the difference in drought tolerance of the two genotypes.
