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Real-time quantitative PCR analysis of human liver biopsies and PBMCs from HBV+ patients, HCV+ patients and healthy donors

ABSTRACT: Real-time quantitative PCR analysis of human liver biopsies and PBMCs from HBV+ patients, HCV+ patients and healthy donors

PROVIDER: PRJNA236491 | ENA |

REPOSITORIES: ENA

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Real-time quantitative PCR analysis of human PBMCs from HCV+ patients and healthy donors

Project description:Human livers biopsies from HCV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RTM-BM-2 ProfilerM-bM-^DM-" PCR ArrayM-BM- was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system. qPCR gene expression profiling. Fresh Livers from 40 HBV+ and 20 HD patients were used . Equal amount total RNA from each samples were used.

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Real-time quantitative PCR analysis of human liver biopsies from HCV+ patients and healthy donors

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Real-time quantitative PCR analysis of human PBMCS from HBV+ patients and healthy donors

Project description:Human livers biopsies from HBV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RTM-BM-2 ProfilerM-bM-^DM-" PCR ArrayM-BM- was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system. qPCR gene expression profiling. PBMCs from 20 HBV+ and 20 HD patients were used . Equal amount total RNA from each samples were used

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Transcriptomic profiling of peripheral blood NK cells of chronic HBV, HCV and HIV patients

Project description:Background: NK cells during chronic viral infection have been well studied over the last decade. We performed an unbiased next-generation RNA-sequencing approach to identify commonalities or differences of the effect of HIV, HCV and HBV viremia on NK cell transcriptomes. Methods: Using cell sorting, we obtained CD3-CD56+ NK cells from blood of 6 HIV, 11 HCV, and 32 HBV infected and untreated patients. Library preparation and sequencing were done using Illumina mRNA-Seq Sample Prep Kit and the HiSeq 2000, HiSeq2500 or NextSeq 500, and further analysis by an in-house analytic pipeline. Results: In NK cells from HIV, HCV and HBV patients, transcriptome analysis identified 272, 53, and 56 differentially expressed genes, respectively (fold change >1.5, q-value 0.2). Interferon stimulated genes were induced in NK cells from HIV/HCV patients, but not during HBV infection. HIV viremia downregulated ribosome assembly genes in NK cells. In HBV, viral load and ALT variation had little effect on genes related to NK effector function. Conclusion: We compare, for the first time, NK cell transcripts of viremic HIV, HCV and HBV patients. We clearly demonstrate distinctive NK cell gene signatures in 3 different populations, suggestive for a different degree of functional alterations of the NK cell compartment as compared to healthy individuals.

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Real-time quantitative PCR analysis of human liver biopsies from HBV+ patients and healthy donors

Project description:Human livers biopsies from HBV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RTM-BM-2 ProfilerM-bM-^DM-" PCR ArrayM-BM- was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system. qPCR gene expression profiling. Fresh Livers from 20 HBV+ and 20 HD patients were used . Equal amount total RNA from each samples were used.

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Project description:Human livers biopsies from HCV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RT² Profiler™ PCR Array was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system.

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Project description:Human livers biopsies from HBV+ patients and healthy donors were collected . RNA and Protein were extract and The Human Adipogenesis RT² Profiler™ PCR Array was used to compare the gene expression in the two group. At the same time PBMCs were collected and the same array were used to compare the adipogenesis expression in the two system.

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Real-time quantitative PCR analysis of human liver biopsies from HCV+ patients and healthy donors

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Real-time quantitative PCR analysis of human liver biopsies from HBV+ patients and healthy donors

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Project description:This SuperSeries is composed of the SubSeries listed below.

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