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Homo sapiens strain:IMR-90

ABSTRACT: Gene expression analysis of human primary lung fibroblast cell line IMR-90 infected by adenovirus type 2

PROVIDER: PRJNA236533 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1146260.fastq.gz	Fastqsanger.gz
	SRR1146261_1.fastq.gz	Fastqsanger.gz
	SRR1146261_2.fastq.gz	Fastqsanger.gz
	SRR1146262_1.fastq.gz	Fastqsanger.gz
	SRR1146262_2.fastq.gz	Fastqsanger.gz

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Phosphoproteome of cyclic stretch A549 and IMR-90

Project description:Lung cancer is one of the leading causes of death. However, most of the researches were based on the traditional cell-culturing method. Whereas cells of lung are subjected to the mechanical forces periodically while breathing. In the present study, we applied cyclic stretch to stimulate the continuously contracting physical condition. We uncovered the stretching force-induced phosphoproteome in lung cancer cell A549 and fibroblast IMR-90. 2048 and 2604 phosphosites corresponding to 837 and 1008 phosphoproteins were identified in A549 and IMR-90, respectively. Interestingly, cytoskeleton reorganization and mitochondrial localization were enriched in the significantly expressed phosphoproteins in response to cyclic stretch. Indeed, we found this physical stress changed cell alignment thus disrupted mitochondrial dynamics. We proved that mitochondrial fusion is induced by uniaxial stretch in 2 cell lines. This study reveals the molecular mechanism of cyclic stretch and supports that stretching force enhanced cellular rearrangement and mitochondrial fusion in lung cells.

2021-09-08 | PXD012010 | Pride

A new look at adenovirus splicing

Project description:Adenovirus type 2 RNA splicing sites were mapped by using deep cDNA sequencing. The majority of the previously identified splice sites were detected. In addition, novel splicing sites were identified Total RNA obtained from four time stages of human primary lung fibroblast cells IMR-90 infected by adenovirus type 2 compared to the control mock-infected by adenovirus type 2.

2014-03-25 | E-GEOD-54583 | biostudies-arrayexpress

Homo sapiens strain:IMR-90

Project description:Small RNA analysis of different time stages of human primary lung fibroblast cells infected by human adenovirus type 2

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NOMe-Seq analysis of IMR-90 cell line

Project description:NOMe-Seq analysis of IMR-90 cell line

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IMR-90 cells: Tissue Culture Polystyrene Time Course

Project description:IMR-90 Human Fibroblasts were seeded onto TCPS dishes. mRNA was isolated at 1, 2, 4, 8, 12, 24, 48 and 96 hours. This time course was used as a control for gene expression studies on collagen/chondroitin sulfate tissue engineering scaffold materials using the same cell line. Keywords = tissue culture plates Keywords: time-course

2003-09-10 | GSE410 | GEO

ChIP-seq from IMR-90 (ENCSR970FKL)

Project description:ChIP-seq on human IMR-90 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-01-04 | GSE92197 | GEO

ChIP-seq from IMR-90 (ENCSR664OKA)

Project description:ELK1 ChIP-seq on human IMR-90 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-01-04 | GSE91969 | GEO

ChIP-seq from IMR-90 (ENCSR124AIG)

Project description:FOS ChIP-seq on human IMR-90 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-10-20 | GSE105300 | GEO

Sequencing of human IMR-90 at 27 and 55 population doublings

Project description:Comparisong of gene expression of human IMR-90 at 27 and 55 population doublings. RNA-seq data comprises 2 groups: 27 and 55 population doublings of IMR-90. Jena Centre for Systems Biology of Ageing - JenAge (www.jenage.de)

2021-10-19 | GSE117813 | GEO

microRNA-seq from IMR-90 (ENCSR123QKC)

Project description:RNA Evaluation IMR-90 microRNA-seq from Mortazavi For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2021-06-15 | GSE176683 | GEO

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