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Homo sapiens strain:IMR-90

ABSTRACT: Small RNA analysis of different time stages of human primary lung fibroblast cells infected by human adenovirus type 2

PROVIDER: PRJNA236786 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1154600.fastq.gz	Fastqsanger.gz
	SRR1154610.fastq.gz	Fastqsanger.gz
	SRR1154601.fastq.gz	Fastqsanger.gz
	SRR1154611.fastq.gz	Fastqsanger.gz
	SRR1154602.fastq.gz	Fastqsanger.gz

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Homo sapiens strain:IMR-90

Project description:Gene expression analysis of human primary lung fibroblast cell line IMR-90 infected by adenovirus type 2

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A new look at adenovirus splicing

Project description:Adenovirus type 2 RNA splicing sites were mapped by using deep cDNA sequencing. The majority of the previously identified splice sites were detected. In addition, novel splicing sites were identified Total RNA obtained from four time stages of human primary lung fibroblast cells IMR-90 infected by adenovirus type 2 compared to the control mock-infected by adenovirus type 2.

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Project description:IMR-90 Human Fibroblasts were seeded onto TCPS dishes. mRNA was isolated at 1, 2, 4, 8, 12, 24, 48 and 96 hours. This time course was used as a control for gene expression studies on collagen/chondroitin sulfate tissue engineering scaffold materials using the same cell line. Keywords = tissue culture plates Keywords: time-course

2003-09-10 | GSE410 | GEO

IMR-90 cells: Collagen Mesh Time Course

Project description:A series of chips with some repeated measurments. Each chip represents a pool of 4 collagen/chondroitin sulfate tissue engineering scaffold meshes seeded with 1 x 10^6 IMR-90 Human Fibroblasts. mRNA was isolated at 30 minutes, 1, 2, 4, 8, 12, 24, 48, and 72 hours after seeding. Keywords = tissue engineering mesh, collagen, chondroitin sulfate, biomaterials design Keywords: time-course

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Phosphoproteome of cyclic stretch A549 and IMR-90

Project description:Lung cancer is one of the leading causes of death. However, most of the researches were based on the traditional cell-culturing method. Whereas cells of lung are subjected to the mechanical forces periodically while breathing. In the present study, we applied cyclic stretch to stimulate the continuously contracting physical condition. We uncovered the stretching force-induced phosphoproteome in lung cancer cell A549 and fibroblast IMR-90. 2048 and 2604 phosphosites corresponding to 837 and 1008 phosphoproteins were identified in A549 and IMR-90, respectively. Interestingly, cytoskeleton reorganization and mitochondrial localization were enriched in the significantly expressed phosphoproteins in response to cyclic stretch. Indeed, we found this physical stress changed cell alignment thus disrupted mitochondrial dynamics. We proved that mitochondrial fusion is induced by uniaxial stretch in 2 cell lines. This study reveals the molecular mechanism of cyclic stretch and supports that stretching force enhanced cellular rearrangement and mitochondrial fusion in lung cells.

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Genome-wide expression profiles of primary human SAECs infected with different adenovirus mutants.

Project description:Full title: Genome-wide expression profiles of primary human small airway epithelial cells (SAECs) infected with different adenovirus mutants. Expression arrays were used to analyze global gene expression changes in SAECs infected with either mock, dE1B-55k, or d55k/dORF3 adenovirus at 36 hours post infection, as well as nutlin treatment for 12 hours. Two independent experiments were performed with each experimental condition done in duplicate. CEL files were imported and analyzed with Partek Analysis software.

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ChIP-seq from IMR-90 (ENCSR197WGI)

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ChIP-seq from IMR-90 (ENCSR970FKL)

Project description:ChIP-seq on human IMR-90 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

2017-01-04 | GSE92197 | GEO

ChIP-seq from IMR-90 (ENCSR957KYB)

Project description:BHLHE40 ChIP-seq on human IMR90 For data usage terms and conditions, please refer to http://www.genome.gov/27528022 and http://www.genome.gov/Pages/Research/ENCODE/ENCODE_Data_Use_Policy_for_External_Users_03-07-14.pdf

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