Project description:All-trans retinoic acid (ATRA) has been shown to have anti-proliferative effects, particularly in the context of cancer. However, the effects of ATRA on gene and microRNA expression in solid tumors have not been investigated. In this study, we performed gene expression and microRNA analysis of the squamous cell carcinoma cell line, ME180, following treatment with 10 micromolar all-trans retinoic acid (ATRA) for 1, 3, and 6 hours. Results provide insight into the temporal regulation of genes and microRNAs by retinoids.
Project description:p63 with and without actinomycin D treatment ChIP from retinoic acid stimulated ME180 Cells at 0 hours using Affymetrix ENCODE tiling chip with NCBI build 35 annotation. p63_ActD and p63_mActD ChIP from retinoic acid stimulated ME180 Cells, 0 hours.
Project description:We evaluated the change in expression of genes after treatment of stellate cells with retinoic acid to understand how the stellate cells can de-differentiate and effect their physiological behaviour in pathological conditions. We then tested the changes in the gene expression in 2D and 3D culture conditions for pancreatic stellate cells and in a pancreatic cancer model. Keywords: gene expression change, time course We treated a pancreatic stellate cell line on plastic with 1 micromolar all-trans retinoic acid (ATRA) for timepoints of 30 mins, 4 hours, 12 hours, 24 hours and 168 hours. RNA was extracted and hybridized on Illumina Human microarrays. We looked for target genes regulated by ATRA and evaluated for time course change.
Project description:Time course experiment for treatment of B16 mouse melanoma cells with all-trans retinoic acid Keywords: Time couse Time-course experiment with treatment at 4hr, 10hr, 24hr, and 48hr. Six biological replicates per time point; one biological replicate per array. Dye swap.
Project description:In vitro time course experiment using the A404 clone of P19 mouse embryonal carcinoma cells to evaluate SMC differentiation in response to retinoic acid followed by phenotype-specific selection with puromycin. Four time points of 5-6 biological replicates each were performed. Keywords: time-course
Project description:To investigate a role of mKIAA genes in early stage of neurite outgrowth, in vitro time course experiment was performed using the mouse neuroblastoma Neuro2A cells and all-trans-retinoic acid. Six time points of 8 biological replicates were performed.These data were normalized by median intensities and subtracted background intensities. NOTE: Outlier involved several data were not deposited. Keywords: time-course
Project description:Targets of Retinoic Acid (RA) were identified in primary human epidermal keratinocytes grown in the presence or absence of all-trans retinoic acid for 1, 4, 24, 48 and 72 hours. Targets of Thyroid Hormone (T3) were identified in primary human epidermal keratinocytes grown in the presence or absence of the hormone; same controls as for RA. Time course, 1, 4, 24, 48 and 72 hours
