Ontology highlight
ABSTRACT:
PROVIDER: PRJNA237183 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Transcriptional profiling of pooled adult kidneys from transgenic zebrafish expressing human KIT-D816V mutant gene versus wild-type zebrafish was performed. The aim of this experiment was to determine the expression and cellular changes caused by expression of KIT-D816V oncogene in the hematopoietic organ in zebrafish. This transgenic zebrafish represents a model of systemic mastocytosis with many features of this disease present in adults
2014-02-03 | GSE54614 | GEO
Project description:Transcriptional profiling of pooled adult kidneys from transgenic zebrafish expressing human KIT-D816V mutant gene versus wild-type zebrafish was performed. The aim of this experiment was to determine the expression and cellular changes caused by expression of KIT-D816V oncogene in the hematopoietic organ in zebrafish. This transgenic zebrafish represents a model of systemic mastocytosis with many features of this disease present in adults Two-condition experiment, Tg(actb2:KIT-D816V) versus wild-type(AB), one replicate of each prepared from 3 adult kidneys of each genotype and a dye-swap hybridization of these RNA samples.
2014-02-03 | E-GEOD-54614 | biostudies-arrayexpress
Project description:Transcriptional profiling of 28 hpf zebrafish transgenic expressing human KIT-D816V mutant gene versus wild-type embryos was performed. The aim of this experiment was to determine the expression changes caused by expression of KIT-D816V oncogene. This transgenic zebrafish represents a model of systemic mastocytosis with many features of this disease present in adults, but no very clear phenotypes in embryos. Thus, we attempted to determine if there are certain expression changes, which can be used as molecular features of active KIT expressed in these embryos.
2014-02-03 | GSE54613 | GEO
Project description:Transcriptional profiling of 28 hpf zebrafish transgenic expressing human KIT-D816V mutant gene versus wild-type embryos was performed. The aim of this experiment was to determine the expression changes caused by expression of KIT-D816V oncogene. This transgenic zebrafish represents a model of systemic mastocytosis with many features of this disease present in adults, but no very clear phenotypes in embryos. Thus, we attempted to determine if there are certain expression changes, which can be used as molecular features of active KIT expressed in these embryos. Two-condition experiment, Tg(actb2:KIT-D816V) versus wild-type(AB), 4 replicate experiments with a dye-swap each time
2014-02-03 | E-GEOD-54613 | biostudies-arrayexpress
Project description:Tg(actb2:KIT-D816V) transgenic zebrafish versus wild-type at 28 hpf
| PRJNA237187 | ENA
Project description:To decipher molecular targets responsible for c-Kit mutant D816V mediated hematopoietic malignancy, we employed whole genome microarray expression profiling as a discovery platform to identify important gene targets downstream of c-Kit mutant D816V expressing cells compared to wild-type c-Kit.
2016-04-30 | GSE76355 | GEO
Project description:Analysis of differentially-expressed genes in Tg(actb2:KIT-D816V) transgenic zebrafish versus wild-type adult kidneys
| PRJNA237185 | ENA
Project description:Aggressive systemic mastocytosis with associated hematologic neoplasm (ASM-AHN) represents a complex myeloid neoplasm, in which KIT D816V is the phenotype-defining but not the only driver mutation. Avapritinib is a potent and specific KIT D816V inhibitor recently approved for ASM treatment. To understand the effects of inhibiting KIT D816V on MC and Non-MC compartments, we combined single cell RNA sequencing (scRNAseq) with deep whole genome sequencing (WGS) to map clonal dynamics in successive blood samples from 4 ASM-AHN patients treated with avapritinib (T1 as pretreatment sample, T2 and T3 as posttreatment samples). Since CMML is the most common AHN associated with ASM, we included samples from three treatment-naïve KIT wild type CMML patients for comparison. We also included 3 age-matched healthy donor as controls.
2024-11-22 | GSE249445 | GEO
Project description:The CD117 mast/stem cell growth factor receptor tyrosine kinase (KIT) is critical for haematopoiesis, melanogenesis, and stem cell maintenance. KIT is commonly activated by mutation in cancers including acute myeloid leukaemia, melanoma, and gastrointestinal stromal tumours (GISTs). The kinase and the juxtamembrane domains of KIT are mutation hotspots; with the kinase domain mutation D816V common in leukaemia, and the juxtamembrane domain mutation V560G common in GISTs. Given the importance of mutant KIT signalling in cancer, we have conducted a proteomic and phosphoproteomic analysis of D816V- and V560G-KIT mutations, using an FDCP1 isogenic cell line model.
2025-06-19 | PXD043783 | Pride
Project description:Cdx2 has been suggested to play an important role in Barrett's esophagus (BE), or intestinal metaplasia (IM) in the esophagus. However, in vivo data have been lacking. The aim of the present study was to investigate whether transgenic overexpression of zCdx1b, the functional equivalent of mammalian Cdx2 in zebrafish, may lead to IM of squamous epithelium in zebrafish A transgenic zebrafish system was developed by expressing zCdx1b gene under the control of zebrafish keratin 5 promoter (zK5p). zCdx1b expression in the esophageal squamous epithelium of transgenic zebrafish was analyzed by in situ hybridization, immunohistochemical staining and RT-PCR. Gene expression in the esophageal squamous epithelium of wild-type and transgenic zebrafish was analyzed by Affymetrix microarray and confirmed by in situ hybridization.
2012-02-18 | GSE35889 | GEO