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Gene expression analysis of airway epithelial cells exposed to flagellin via RNA-seq and microarray

ABSTRACT: Gene expression analysis of airway epithelial cells exposed to flagellin via RNA-seq and microarray

PROVIDER: PRJNA239683 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1179090.fastq.gz	Fastqsanger.gz
	SRR1179100.fastq.gz	Fastqsanger.gz
	SRR1179110.fastq.gz	Fastqsanger.gz
	SRR1179120.fastq.gz	Fastqsanger.gz
	SRR1179130.fastq.gz	Fastqsanger.gz

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Gene expression analysis of airway epithelial cells exposed to flagellin via microarray

Project description:Airway epithelial cells (AEC) are critical components of the inflammatory and immune response during exposure to pathogens. AECs in monolayer culture and differentiated epithelial cells in air-liquid interface (ALI) represent two distinct and commonly used in vitro models, yet differences in their response to pathogens have not been investigated. In this study, we compared the transcriptional effects of flagellin on AECs in monolayer culture versus ALI culture using exon microarrays and RNAsequencing. We found that AECs cultured in monolayer and ALI have strikingly different transcriptional states at baseline. When challenged with flagellin, monolayer AEC cultures greatly increased transcription of numerous genes mapping to wounding response, immunity and inflammatory response. In contrast, AECs in ALI culture had an unexpectedly muted response to flagellin, both in number of genes expressed and relative enrichment of inflammatory and immune pathways. In conclusion, In vitro culturing methods have a dramatic effect on the transcriptional profile of AECs at baseline and after stimulation with flagellin. These differences suggest that epithelial responses to pathogen challenges are distinctly different in culture models of intact and injured epithelium.

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Gene expression analysis of airway epithelial cells exposed to flagellin via RNA-seq

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Project description:Here, we aim to understand the role of P38 in the airway epithelial cells response to flagellin. We report the gene expression profile of pig airway epithelial cells cultured under air-liquid conditions, pre-incubated or not with the P38 inhibitor SB203580 (20 µM, Tocris Bioscience) for 1 h and then stimulated with 100 ng/ml of a mutated flagellin (FliCΔ174-400) for 2h or 24h. Our data show that flagellin stimulation induce the expression of pro-inflammatory cytokines mainly through the NFkB pathway, with little impact of P38 inhibition in the cells response.

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Single-cell transcriptomics reveals subset-specific metabolic profiles underpinning the bronchial epithelial response to flagellin

Project description:Airway epithelial cells represent the first line of defense against respiratory pathogens. Flagellin drives the motility of many mucosal pathogens and has been suggested as an immune enhancing adjunctive therapeutic in infections of the airways. This study leveraged single-cell RNA sequencing to determine cell-specific effects of flagellin in primary human bronchial epithelial cells growing in air-liquid interface. Seven cell clusters were identified, including ciliated cells, ionocytes and several states of basal and secretory cells, of which only inflammatory basal cells and inflammatory secretory cells demonstrated a proportional increase in response to flagellin. Inflammatory secretory cells showed evidence of metabolic reprogramming toward aerobic glycolysis, whilst in inflammatory basal cells transcriptome profiles indicated enhanced oxidative phosphorylation. Inhibition of mTOR prevented the shift to glycolysis and reduced inflammatory gene transcription specifically in inflammatory secretory cells. This data demonstrates the functional heterogeneity of the human airway epithelium upon exposure to flagellin.

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Gene expression analysis of airway epithelial cells exposed to flagellin via RNA-seq and microarray

Project description:This SuperSeries is composed of the SubSeries listed below.

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Project description:RNAseq was used to measure the transciptional respose of human bronchial epithelium (HBE) to the bacterial component flagellin with particular emphasis on innate immunity and metabolism.

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