ABSTRACT: Using DNA microarray to screen intracellular signal pathways of hypoglycemic activity by electroacupuncture of Zusanli (ST36) acupoints in streptozotocin-induced rats with diabetes
Project description:The aim of this study is to screen the possible effective intracellular signaling pathways of EA on bilateral Zusanli acupoint (ST36) by DNA microarray in rats with diabetes. Streptozotocin (STZ) rats with diabetes were randomly assigned to the experimental (EA) group or the control (non-EA) group. The exploratory methods for analysis in this study were the following points: (i) detecting plasma glucose levels (ii) pathway analysis by DNA microarray in rats' gastrocnemius muscle. The pathway analysis of DNA microarray showed that the insulin signaling pathway, PPAR signaling pathway, MAPK signaling pathway or Wnt signal pathway might be related to the hypoglycemic effect of STZ rats with diabetes induced by EA. Using DNA microarray would be helpful in screening the possible targets of signaling pathways. The hypoglycemic effect of EA was close relative to insulin and relative intracellular signal pathways. Further research will be required to determine the details of the intracellular signaling pathways of the hypoglycemic effect induced by EA.
Project description:We performed microarray miRNA expression profiling of diabetes induced rat via intraperitoneal (I.P) administration of streptozotocin (STZ). Rats were considered diabetic when their blood glucose exceeded 200 mg/dL (11 mmol/L).
Project description:Gene expression analysis in control and diabetic rats. Diabetes-induced erectile dysfunction in rat model of DM. 10 weeks of streptozotocin induced diabetes. F344 Rats. Keywords: other
Project description:Myocardial compensatory mechanisms stimulated by reduced oxygen utilization caused by streptozotocin-induced diabetes mellitus (D) and treated with the dichloroacetate (DCA) are presumably associated with the regulation of mitochondria. We aimed to promote the understanding of key signaling pathways and identify effectors involved in signal transduction.
Project description:Fisher male Cyp1a1mRen2 rats were allocated into four groups of four biological replicates each: control; streptozotocin-induced diabetes alone; indole-3-carbinol induced hypertension alone; combined induced diabetes and hypertension. <br>Kidneys were harvested for microarray analysis.analysis.
Project description:Gene expression analysis in control and diabetic rats. Diabetes-induced erectile dysfunction in rat model of DM. 10 weeks of streptozotocin induced diabetes. F344 Rats.
Project description:The mitochondria-associated endoplasmic reticulum (ER) membrane (MAM) is the physical contact site between the ER and the mitochondria and plays a vital role in the regulation of calcium trafficking and bioenergetics. Previous studies suggest that disturbances in calcium trafficking and dysregulation of reactive oxygen species in the ER and mitochondria may contribute to the pathogenesis of diabetic retinopathy (DR). However, few studies have examined the impact of diabetes on the retinal MAM proteome profile. In the present study, we used Long Evans rats with streptozotocin-induced long-term Type 1 diabetes to identify key pathways and proteins in the retinal MAM that are potentially implicated in the pathogenesis of DR.
Project description:A study of diabetic neuropathy in dorsal root ganglia from streptozotocin-diabetic male wistar rats over the first 8 weeks of diabetes
Project description:Anaesthesia time was varied between 4 hours and a quick 5 min sacrifice in streptozotocin diabetic rats and controls after four weeks of diabetes.
Project description:Effect of type 1 diabetes (induced by streptozotocin 60 mg/kg) on lung gene expression. Wistar rats, male. At age 8 weeks control rats got IP buffer, diabetic rats got streptozotocin. At age 12 weeks animals were anesthetized and lungs removed. RNA was extracted with Trizol, and gene expression array analysis was performed using Affymetrix RAE 230A microarrays according to the directions from the manufacturer. Arrays were scanned using a Hewlett Packard Gene Array scanner, and analyzed with Affymetrix MAS 5.0 software. Expression levels reported are the output from the MAS software.
