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Schizosaccharomyces pombe

ABSTRACT: Role of Fun30 remodelers in retrotransposon regulation [MNase-Seq]

PROVIDER: PRJNA245877 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1262281_1.fastq.gz	Fastqsanger.gz
	SRR1262281_2.fastq.gz	Fastqsanger.gz
	SRR1262282_1.fastq.gz	Fastqsanger.gz
	SRR1262282_2.fastq.gz	Fastqsanger.gz
	SRR1262283_1.fastq.gz	Fastqsanger.gz

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Schizosaccharomyces pombe

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Project description:Chromatin remodeler Fun30-Fft3 disassembles nucleosomes to facilitate RNAPII elongation in fission yeast

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Role of Fft3 in nuclear organization [MNase-seq]

Project description:In eukaryotic cells, local chromatin structure and chromatin organization in the nucleus both influence transcriptional regulation. At the local level, the Fun30 chromatin remodeler Fft3 is essential for maintaining proper chromatin structure at centromeres and subtelomeres in fission yeast. Using genome-wide mapping and live cell imaging, we show that this role is linked to controlling nuclear organization of its targets. In fft3M-NM-^T cells, subtelomeres lose their association with the LEM domain protein Man1 at the nuclear periphery and move to the interior of the nucleus. Furthermore, genes in these domains are upregulated and active chromatin marks increase. Fft3 is also enriched at retrotransposon-derived long terminal repeat (LTR) elements at the borders of subtelomeres and at tRNA genes. In cells lacking Fft3, these sites lose their peripheral positioning and show reduced nucleosome occupancy. We propose that Fft3 has a global role in mediating association between specific chromatin domains and components of the nuclear envelope by maintaining chromatin structure required for anchoring DNA insulators to nuclear pores. For MNase samples, duplicate mutant mononucleosome fractions were compared with duplicate WT mononucleosomes.

2015-03-02 | E-GEOD-58012 | biostudies-arrayexpress

Schizosaccharomyces pombe

Project description:Hrp3 controls nucleosome positioning to suppress non-coding transcription in eu- and heterochromatin [MNase-Seq]

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Project description:Spt6 regulates intragenic and antisense transcription, nucleosome positioning, and histone modifications genome-wide in fission yeast [Mnase-seq]

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