Project description:Drosophila PTB (Polypyrimidine Tract-binding protein dmPTB) regulates dorso-ventral patterning genes in embryos Comparison of wild type (yw genotype) and PTB mutant (heph03429) drosophila embryos
Project description:The aim of this study was to analyze if transcriptionally active svb loci show enrichment for H3K4me1. To capture cells where svb-related ventral enhancers are active, we FACS-sorted nuclei from stage 15 Drosophila melanogaster embryos based on the expression of different reporter genes driven by different svb enhancers. The svb_E10 enhancer drives the expression of GFP, whilst dsRed is driven by the svb_7 enhancer. The sorted nuclei were then used for ChIP with anti-H3K4me1 and anti-H3 (for normalization purposes) antibodies.