Arabidopsis thaliana
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA248481 | ENA |
REPOSITORIES: ENA
Similar Datasets
Project description:Direct roles of SPEECHLESS in the specification of stomatal self-renewing cells [ChIP-Seq]
| PRJNA246551 | ENA
Project description:Nanopore direct RNA-seq for Arabidopsis thaliana
| PRJNA741703 | ENA
Project description:FLP and MYB88 are two paralogous MYB proteins, regulating the symmetric division of guard mother cell during Arabidopsis stomatal development. To understand their molecular functions, we performed genome-wide identification of FLP/MYB88 binding targets using ChIP-chip with FLP/MYB88 antibody. By comparing ChIP-chip between wild-type and flp-1 myb88 lines, a total genes were identified as putative direct targets for FLP/MYB88.
2010-09-01 | GSE19763 | GEO
Project description:Molecular profiling of stomatal lineage cell states
| PRJNA140931 | ENA
Project description:Stomata are highly specialized organs which consist of pairs of guard cells and regulate gas and water vapor exchange in plants. While early stages of guard cell differentiation have been described and were interpreted in analogy to processes of cell type differentiation in animals, the downstream development of functional stomatal guard cells remains poorly understood. We have isolated an Arabidopsis mutant, scap1 (stomatal carpenter 1), that develops irregularly shaped guard cells and lacks the ability to control stomatal aperture, including CO2-induced stomatal closing and light-induced stomatal opening. SCAP1 was identified as a plant-specific Dof-type transcription factor expressed in maturing guard cells but not in guard mother cells. SCAP1 regulates the expression of genes encoding key elements of stomatal functioning and morphogenesis, such as a K+ channel protein, MYB60 transcription factor, and pectin methyl esterase. Consequently, ion homeostasis was disturbed in scap1 guard cells, and esterification of extracellular pectins was impaired so that the cell walls lining the pores did not mature normally. We conclude that SCAP1 regulates essential processes of stomatal guard cell maturation and functions as a key transcription factor regulating the final stages of guard cell differentiation.
2013-02-28 | GSE43964 | GEO
Project description:To gain initial insight into the regulatory mechanisms by which signalling in response to an elevated CO2 concentration exerts CA1- and CA4-dependent repression of stomatal development, we conducted high-throughput RNA-seq transcriptomics on immature aerial tissues of A. thaliana seedlings grown at the low (150 p.p.m) and elevated CO2 concentrations (500 p.p.m). Hypocotyls and cotyledons of developing seedlings (5 DAG; WT and ca1 ca4 mutant plants; n>1,000 per sample) grown in the low and elevated CO2 concentrations were used as source tissue to extract total RNA and conduct RNA-seq experiments using the HiSeq 2000 platform (Illumina). The raw data from three independent biological replicates (experiments).
2014-09-24 | E-MTAB-4447 | biostudies-arrayexpress
Project description:FLP and MYB88 are two paralogous MYB proteins, regulating the symmetric division of guard mother cell during Arabidopsis stomatal development. To understand their molecular functions, we performed genome-wide identification of FLP/MYB88 binding targets using ChIP-chip with FLP/MYB88 antibody. By comparing ChIP-chip between wild-type and flp-1 myb88 lines, a total genes were identified as putative direct targets for FLP/MYB88. Ten-day old wild-type and flp-1 myb88 Arabidopsis green tissues were used for ChIP. Rabbit polyclonal antibody recognizing FLP and MYB88 proteins was used for chromatin immunoprecipitation
2010-08-31 | E-GEOD-19763 | biostudies-arrayexpress
Project description:Extensively Self Renewing Erythroblasts (ESREs) are cultured erythroid cells derived from yolk sack or fetal liver. Transcriptome analyses were conducted on ESREs using RNA-seq and compared to published transcriptome analyses of uncultured erythroid cells. RNA-seq was conducted on proliferating Extensively Self Renewing Erythroblasts (ESREs) and ESREs after one day of maturation. Biologic duplicates were done at each time point.
2014-10-08 | E-GEOD-54826 | biostudies-arrayexpress
Project description:Stomata are highly specialized organs which consist of pairs of guard cells and regulate gas and water vapor exchange in plants. While early stages of guard cell differentiation have been described and were interpreted in analogy to processes of cell type differentiation in animals, the downstream development of functional stomatal guard cells remains poorly understood. We have isolated an Arabidopsis mutant, scap1 (stomatal carpenter 1), that develops irregularly shaped guard cells and lacks the ability to control stomatal aperture, including CO2-induced stomatal closing and light-induced stomatal opening. SCAP1 was identified as a plant-specific Dof-type transcription factor expressed in maturing guard cells but not in guard mother cells. SCAP1 regulates the expression of genes encoding key elements of stomatal functioning and morphogenesis, such as a K+ channel protein, MYB60 transcription factor, and pectin methyl esterase. Consequently, ion homeostasis was disturbed in scap1 guard cells, and esterification of extracellular pectins was impaired so that the cell walls lining the pores did not mature normally. We conclude that SCAP1 regulates essential processes of stomatal guard cell maturation and functions as a key transcription factor regulating the final stages of guard cell differentiation. We isolated guard cell protoplasts from 4-week-old WT(Col-0) and scap1 mutant plants and extracted RNA independently. Four biological replicates were performed for each experiment.
2013-02-28 | E-GEOD-43964 | biostudies-arrayexpress