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Homo sapiens

ABSTRACT: Flag-RBM47 HITS-CLIP in brain metastatic derivatives of MDA-MB-231

OTHER RELATED OMICS DATASETS IN: E-GEOD-58379

PROVIDER: PRJNA252382 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	SRR1374807.fastq.gz	Fastqsanger.gz
	SRR1374808.fastq.gz	Fastqsanger.gz

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Flag-RBM47 HITS-CLIP in brain metastatic derivatives of MDA-MB-231

Project description:Brain metastatic breast cancer cells were subjected to HITS-CLIP to identify the targets of the RNA binding protein RBM47 MDA231-BrM2a is characterized in PMID: 19421193; Related data published together with these data are found in GSE53779 Non-clonal brain metastatic breast cancer cells stably expressing Flag-tagged, wild-type RBM47 under a doxycline-inducible promoter were treated for three days with doxycycline then UV-irradiated and subjected to Flag HITS-CLIP

2014-06-11 | E-GEOD-58380 | biostudies-arrayexpress

MBNL1 HITS-CLIP in MDA-MB-231 breast cancer cells

Project description:HITS-CLIP was performed to identify endogenous MBNL1 binding sites on RNAs in MDA-MB-231 breast cancer cells.

2016-10-14 | GSE76486 | GEO

Nuclear HNRNPA2B1 HITS-CLIP and RNA-seq

Project description:To identify HNRNPA2B1 binding sites on endogenous nuclear RNAs, we performed HITS-CLIP for endogenous HNRNPA2B1 and RNA-seq to analyze the nuclear RNA under either METTL3 or HNRNPA2B1 depletion. Wild type MDA-MB-231 cells were subjected to the HITS-CLIP procedure on immunoprecipitated HNRNPA2B1 associated RNA obtained from the nuclear fraction (Licatalosi D, et al. 2008, Nature 456:464-U22). For RNA-seq, nuclear RNA was extracted from MDA-MB-231 or Hela cells knocked down for METTL3 or HNRNPA2B1.

2015-06-20 | E-GEOD-70061 | biostudies-arrayexpress

Flag-RBM47 HITS-CLIP in brain metastatic derivatives of MDA-MB-231

2014-06-11 | GSE58380 | GEO

Homo sapiens strain:MDA-MB-231

Project description:Human MDA-MB-231 Cell HITS-CLIP RNA sequencing

| PRJNA257235 | ENA

Homo sapiens

Project description:MBNL1 HITS-CLIP in MDA-MB-231 breast cancer cells

| PRJNA307558 | ENA

Identification of circRNA Expression Signatures for breast cancer metastasis

Project description:To identify breast cancer metastasis-relevant circRNAs, we assessed the expression profiles of circRNAs in parental MDA-MB-231 (231-PAR) cells, isogenic brain metastatic cells (231-BM6), lung metastatic cells (LM2) and bone metastatic cells (1833), which were isolated from brain, lung or bone-seeking 231-PAR cells

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Hybrids assimilate organ-specific metastasis gene signatures from both parental cells

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Transcription profiling of human parental parental line MDA-MB-231 and fusion cell lines indicates hybrids assimilate organ-specific metastasis gene signatures from both parental cells

Project description:Spontaneous cell fusion of MDA-MB-231 bone-metastatic subline Bm (i.e., SCP2) and lung metastatic subline Lm (i.e., LM2) gave rise to hybrid lines BLm-FACS or BLm-DRUG, as well as its single clones (#8, #12, #18). The hybrids acquired the metastasis tropisms from both parental cells. Expression profiles of the parental cells, the hybrids and several previously characterized MDA-MB-231 metastatic derivatives were compared. Hierarchical clustering showed the hybrids assimilated the organ-specific metastasis gene signatures from both parental cells. Experiment Overall Design: Twenty-six cell lines were analyzed, including the parental line MDA-MB-231; cell fusion partner lines Bm and Lm; self-fused lines BBm and LLm; hetero-fused lines BLm-FACS, BLM-DRUG and clones BLm-DRUG-8, -12 and -18; strongly bone-metastatic lines 1833, SCP14, SCP20, SCP25 and SCP46; strongly lung-metastatic lines 3481, 4142, 4173, 4175 and 4180; and weakly metastatic lines SCP3, SCP4, SCP6, SCP28, SCP32 and SCP43. Single sample for each line.

2009-05-16 | E-GEOD-14244 | biostudies-arrayexpress

Gene expression profile in breast cancer cells

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