Project description:The goals of this study are to compare transcriptome profiling (RNA-seq) of bone marrow derived macrophages from wild-type and PHLPP1 knock-out mice and how Inflammatory stimulation affects those profiles.
Project description:Bone marrow-derived macrophages from mice were treated with recombinant Ssa1, a protein enriched in the hypoxic secretome of Candida albicans.
Project description:To characterize the genome wide C/EBPepsilon binding sites, ChIP-sequencing with C57BL/6 mice bone marrow cells were performed. To prevent artifact from PCR amplifiation, sequencing or antibody specificity, two controls of input DNA and ChIP-sequencing of C/EBPepsilon knock-out bone marrow cells were used. Two data sets derived from two anti-C/EBPepsilon antibodies, N-terminal (GTX109155) and C-terminal (SC-158) antibody against wild type and C/EBPepsilon knock-out bone marrow cells were generated.
Project description:Dendritic cells isolated from murine bone marrow were cultured for 7 days and then stimulated with 100 ng/mL LPS for 24h. Cells were derived from either wildtype or microRNA-146a knock-out mice. Total RNA was isolated from the cells following stimulation.
Project description:In this dataset we include the data obtained from 3 hour stimulation with Neisseria gonorrhoeae (GC) of bone marrow macrophages(BMDM) from wild type (C57BL/6) and Nod2 knock out mice (in C57BL/6 background).
Project description:Dendritic cells isolated from murine bone marrow were cultured for 7 days and then stimulated with 50 ng/mL LPS and 5 mM ATP. Cells derived from both wild type and microRNA-155 knock-out mice. Total RNA was isolated from the cells following stimulation.
