Mus musculus
Ontology highlight
ABSTRACT:
PROVIDER: PRJNA253628 | ENA |
REPOSITORIES: ENA
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| SRR1462280.fastq.gz | Fastqsanger.gz | |||
| SRR1462281.fastq.gz | Fastqsanger.gz | |||
| SRR1462282.fastq.gz | Fastqsanger.gz | |||
| SRR1462283.fastq.gz | Fastqsanger.gz | |||
| SRR1462284.fastq.gz | Fastqsanger.gz |
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Project description:Bicaudal C1 KO embryonic pancreas develops cysts and has less endocrine progenitors after E14.5 (14.5 days after fertilization), while no defect is observed at E13.5. Bicaudal C1 is an RNA-binding protein. To understand the molecular mechanisms leading to both phenotypes, the mRNA expression profile of E13.5 WT vs. Bicaudal C1 dorsal pancreas was studied by high-throughput sequencing using the Illumina HiSeq 2000 platform. This time point was selected as a time point where no phenotypic modification was detected. 3 replicates for each condition were sequenced and each replicate consisted of 3 E13.5 dorsal pancreas of the same genotype. There were only few differences between both transcriptomes. Pkd2 was 1.9-fold reduced in Bicaudal C1 KO. Pkd2 inactivation causes renal and pancreatic cyst. Some genes having immune/inflammatory functions were up or downregulated highlighting the early immune cell infiltration observed in Bicaudal C1 KO pancreas. No hit could explain the endocrine progenitor decrease. It may be due to the ability of Bicaudal C1 to regulate mRNA translation without affecting the mRNAs themselves. Comparison of mRNA profiles of E13.5 WT vs. Bicaudal C1 KO dorsal pancreas using the Illumina HiSeq 2000 platform (platform ID SN865), 3 replicates per condition.
2015-04-07 | E-GEOD-58833 | biostudies-arrayexpress
Project description:Bicaudal C1 KO embryonic pancreas develops cysts and has less endocrine progenitors after E14.5 (14.5 days after fertilization), while no defect is observed at E13.5. Bicaudal C1 is an RNA-binding protein. To understand the molecular mechanisms leading to both phenotypes, the mRNA expression profile of E13.5 WT vs. Bicaudal C1 dorsal pancreas was studied by high-throughput sequencing using the Illumina HiSeq 2000 platform. This time point was selected as a time point where no phenotypic modification was detected. 3 replicates for each condition were sequenced and each replicate consisted of 3 E13.5 dorsal pancreas of the same genotype. There were only few differences between both transcriptomes. Pkd2 was 1.9-fold reduced in Bicaudal C1 KO. Pkd2 inactivation causes renal and pancreatic cyst. Some genes having immune/inflammatory functions were up or downregulated highlighting the early immune cell infiltration observed in Bicaudal C1 KO pancreas. No hit could explain the endocrine progenitor decrease. It may be due to the ability of Bicaudal C1 to regulate mRNA translation without affecting the mRNAs themselves.
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Project description:Effect of fbw7 deletion in mouse pancreatic ducts
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Project description:PURPOSE: To provide a detailed gene expression profile of the normal postnatal mouse cornea. METHODS: Serial analysis of gene expression (SAGE) was performed on postnatal day (PN)9 and adult mouse (6 week) total corneas. The expression of selected genes was analyzed by in situ hybridization. RESULTS: A total of 64,272 PN9 and 62,206 adult tags were sequenced. Mouse corneal transcriptomes are composed of at least 19,544 and 18,509 unique mRNAs, respectively. One third of the unique tags were expressed at both stages, whereas a third was identified exclusively in PN9 or adult corneas. Three hundred thirty-four PN9 and 339 adult tags were enriched more than fivefold over other published nonocular libraries. Abundant transcripts were associated with metabolic functions, redox activities, and barrier integrity. Three members of the Ly-6/uPAR family whose functions are unknown in the cornea constitute more than 1% of the total mRNA. Aquaporin 5, epithelial membrane protein and glutathione-S-transferase (GST) omega-1, and GST alpha-4 mRNAs were preferentially expressed in distinct corneal epithelial layers, providing new markers for stratification. More than 200 tags were differentially expressed, of which 25 mediate transcription. CONCLUSIONS: In addition to providing a detailed profile of expressed genes in the PN9 and mature mouse cornea, the present SAGE data demonstrate dynamic changes in gene expression after eye opening and provide new probes for exploring corneal epithelial cell stratification, development, and function and for exploring the intricate relationship between programmed and environmentally induced gene expression in the cornea. Keywords: other
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Project description:We created mice, which are deficient for Myc specifically in cardiac myocytes by crossing crossed Myc-floxed mice (Mycfl/fl) and MLC-2VCre/+ mice. Serial analysis of earlier stages of gestation revealed that Myc-deficient mice died prematurely at E13.5-14.5. Morphological analyses of E13.5 Myc-null embryos showed normal ventricular size and structure; however, decreased cardiac myocyte proliferation and increased apoptosis was observed. BrdU incorporation rates were also decreased significantly in Myc-null myocardium. Myc-null mice displayed a 3.67-fold increase in apoptotic cardiomyocytes by TUNEL assay. We examined global gene expression using oligonucleotide microarrays. Numerous genes involved in mitochondrial death pathways were dysregulated including Bnip3L and Birc2. Hearts were taken from wide type and Myc-null Mouse embryos at E13.5 under the dissecting scope. Cardiac myocyte RNA was isolated using TRIZOL®Reagent Total RNA (100 ng) was hybridized to the Sentrix® MouseRef-8 Expression BeadChip that contains probes for ~24,000 transcripts. GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G2500A. The data were analyzed with Illumina Inc. BeadStudio version 1.5.0.34 and normalized by rank invariant method.
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